Possible compensation in epidermal growth factor production by saliva in rat

J. Blazsek, K. Offenmüller, B. Burghardt, I. Kisfalvi, K. Birki, M. Wenczl, G. Varga, T. Zelles

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the present work, the authors investigated the effect of both main vegetative transmitters and some regulatory peptides on epidermal growth factor (EGF) secretion into whole saliva. We studied how the salivary and glandular EGF levels depend on the functional activity of salivary glands in rats. Experimental groups comprised: (a) control, (b) bilateral ablation (AB) of submandibular and sublingual salivary glands, (c) 0.5% citric acid in drinking water treatment (Cit), and (d) ablation plus citric acid combination (AB+Cit). On the 7th day under narcosis, pilocarpine-stimulated saliva was collected and the amylase activity was measured along with concentrations of EGF and the protein, both in saliva and in salivary glands. In the first study, in conscious rats adrenergic activation of EGF secretion was observed. During noradrenaline stimulation, the curve showing EGF discharge was parallel to the amylase secretory curve. Salivary amylase secretion was stimulated by adrenergic, cholinergic and VIP-ergic actions. Other regulatory peptides, such as CCK, bombesin and somatostatin, did not seem to be involved in controlling EGF or amylase secretion. In the second study, 7 days after ablation, protein concentration of salivary glands decreased in control animals 30 min after pilocarpine stimulation. There was no significant change in protein concentration after this stimulation in the other groups. EGF concentration increased about 40% in submandibular tissues of the Cit group of animals, and decreased in the parotid tissues in all treated groups. The EGF concentration decreased after pilocarpine stimulation to a great extent in all salivary tissues. Protein concentration in saliva was significantly higher than the initial level in all treated groups (AB:+240%; Cit: +80%; AB+Cit: +350%). EGF concentration of saliva was slightly decreased in the ablation group (-10%), while it increased in the other treated animals (Cit: +20%, AB+Cit: +200%). Changes in the EGF level in saliva were lower than 10% in the control group. In conclusion: 1. EGF secretion is controlled by adrenergic pathways but not by cholinergic mechanisms or by VIP, CCK, bombesin or somatostatin, 2. The EGF level in saliva can be increased by enhanced activity of salivary glands, 3. EGF in saliva is not exclusively produced by one type of salivary glands, 4. Parotid glands can compensate the EGF secretion to saliva when functional activity of the other main gland is lost.

Original languageEnglish
Pages (from-to)279-295
Number of pages17
JournalInflammopharmacology
Volume4
Issue number3
DOIs
Publication statusPublished - Sep 1996

Fingerprint

Saliva
Epidermal Growth Factor
Salivary Glands
Amylases
Pilocarpine
Adrenergic Agents
Bombesin
Somatostatin
Citric Acid
Cholinergic Agents
Sublingual Gland
Salivary Proteins and Peptides
Stupor
Peptides
Proteins
Water Purification
Parotid Gland
Drinking Water
Norepinephrine

Keywords

  • ablation
  • bombesin
  • CCK
  • citric acid
  • epidermal growth factor
  • functional compensation
  • gustatoric stimulus
  • regulatory peptides
  • salivary glands
  • somatostatin
  • VIP

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Immunology

Cite this

Possible compensation in epidermal growth factor production by saliva in rat. / Blazsek, J.; Offenmüller, K.; Burghardt, B.; Kisfalvi, I.; Birki, K.; Wenczl, M.; Varga, G.; Zelles, T.

In: Inflammopharmacology, Vol. 4, No. 3, 09.1996, p. 279-295.

Research output: Contribution to journalArticle

Blazsek, J. ; Offenmüller, K. ; Burghardt, B. ; Kisfalvi, I. ; Birki, K. ; Wenczl, M. ; Varga, G. ; Zelles, T. / Possible compensation in epidermal growth factor production by saliva in rat. In: Inflammopharmacology. 1996 ; Vol. 4, No. 3. pp. 279-295.
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abstract = "In the present work, the authors investigated the effect of both main vegetative transmitters and some regulatory peptides on epidermal growth factor (EGF) secretion into whole saliva. We studied how the salivary and glandular EGF levels depend on the functional activity of salivary glands in rats. Experimental groups comprised: (a) control, (b) bilateral ablation (AB) of submandibular and sublingual salivary glands, (c) 0.5{\%} citric acid in drinking water treatment (Cit), and (d) ablation plus citric acid combination (AB+Cit). On the 7th day under narcosis, pilocarpine-stimulated saliva was collected and the amylase activity was measured along with concentrations of EGF and the protein, both in saliva and in salivary glands. In the first study, in conscious rats adrenergic activation of EGF secretion was observed. During noradrenaline stimulation, the curve showing EGF discharge was parallel to the amylase secretory curve. Salivary amylase secretion was stimulated by adrenergic, cholinergic and VIP-ergic actions. Other regulatory peptides, such as CCK, bombesin and somatostatin, did not seem to be involved in controlling EGF or amylase secretion. In the second study, 7 days after ablation, protein concentration of salivary glands decreased in control animals 30 min after pilocarpine stimulation. There was no significant change in protein concentration after this stimulation in the other groups. EGF concentration increased about 40{\%} in submandibular tissues of the Cit group of animals, and decreased in the parotid tissues in all treated groups. The EGF concentration decreased after pilocarpine stimulation to a great extent in all salivary tissues. Protein concentration in saliva was significantly higher than the initial level in all treated groups (AB:+240{\%}; Cit: +80{\%}; AB+Cit: +350{\%}). EGF concentration of saliva was slightly decreased in the ablation group (-10{\%}), while it increased in the other treated animals (Cit: +20{\%}, AB+Cit: +200{\%}). Changes in the EGF level in saliva were lower than 10{\%} in the control group. In conclusion: 1. EGF secretion is controlled by adrenergic pathways but not by cholinergic mechanisms or by VIP, CCK, bombesin or somatostatin, 2. The EGF level in saliva can be increased by enhanced activity of salivary glands, 3. EGF in saliva is not exclusively produced by one type of salivary glands, 4. Parotid glands can compensate the EGF secretion to saliva when functional activity of the other main gland is lost.",
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AU - Burghardt, B.

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AU - Birki, K.

AU - Wenczl, M.

AU - Varga, G.

AU - Zelles, T.

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