Possible causes of the loss of specific pBR322-Ad h 1 DNA recombinants following transfection.

L. Palkonyay, G. Berencsi, P. Geck, I. Nász

Research output: Contribution to journalArticle

Abstract

Characteristics of pBR322/Ad h 1 DNA recombinants were studied which had been cloned using HindIII restriction endonuclease in a single "shot-gun" experiment. Both oxytetracycline and ampicillin resistance of the clones were found to be heterogeneous. Ad h 1 DNA fragments HindIII-A, and -C could be cloned only in combination with other fragments. Among the possible reasons of the loss of recombinants upon transfection the impairment of pBR322-specific gene functions by the inserts is discussed in addition to the increased tetracycline resistance, and the tertiary structure of recombinant DNA.

Original languageEnglish
Pages (from-to)179-188
Number of pages10
JournalActa microbiologica Hungarica
Volume30
Issue number3-4
Publication statusPublished - Dec 1 1983

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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