Possible causes of the loss of specific pBR322-Ad h 1 DNA recombinants following transfection.

L. Palkonyay, G. Berencsi, P. Geck, I. Nász

Research output: Contribution to journalArticle

Abstract

Characteristics of pBR322/Ad h 1 DNA recombinants were studied which had been cloned using HindIII restriction endonuclease in a single "shot-gun" experiment. Both oxytetracycline and ampicillin resistance of the clones were found to be heterogeneous. Ad h 1 DNA fragments HindIII-A, and -C could be cloned only in combination with other fragments. Among the possible reasons of the loss of recombinants upon transfection the impairment of pBR322-specific gene functions by the inserts is discussed in addition to the increased tetracycline resistance, and the tertiary structure of recombinant DNA.

Original languageEnglish
Pages (from-to)179-188
Number of pages10
JournalActa Microbiologica Hungarica
Volume30
Issue number3-4
Publication statusPublished - 1983

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Recombinant DNA
Transfection
Ampicillin Resistance
Tetracycline Resistance
Oxytetracycline
DNA Restriction Enzymes
Firearms
Clone Cells
DNA
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Possible causes of the loss of specific pBR322-Ad h 1 DNA recombinants following transfection. / Palkonyay, L.; Berencsi, G.; Geck, P.; Nász, I.

In: Acta Microbiologica Hungarica, Vol. 30, No. 3-4, 1983, p. 179-188.

Research output: Contribution to journalArticle

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