Possibilities for fertility restoration

A new surgical technique

Krishen Sieunarine, Faris B P Zakaria, Deborah C M Boyle, David J. Corless, David E. Noakes, Iain Lindsay, Andrew Lawson, L. Ungár, Giuseppe Del Priore, J. Richard Smith

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Previously published work concluded that uterine artery microvascular anastomosis in the porcine model was feasible with subsequent normal vascular function in pregnancy of the anastomosed vessels. The objective of this study was to assess the feasibility of uterine autotransplantation in a porcine model using microvascular anastomoses. Eight large white/landrace sows of proven fertility were used. A supracervical hysterectomy with or without bilateral salpingo-oophorectomy (BSO) was performed. After 1 hour of cold storage in a transplant solution, the specimen was reintroduced and followed by stepwise vascular reanastomosis. Objective perfusion index measurements suggested adequate uterine perfusion after transplantation. Postoperatively, sow 1 survived to 3 months with no signs of oestrus, and sows 2, 6, and 8 were killed on days 6, 33, and 54, respectively, for pelvic infection. Histopathology of the uterine grafts revealed gradual vessel thromboses. Microvascular reanastomosis is feasible, however, unsuccessful in uterine autotransplantation because of gradual vessel thromboses. The porcine model is highly susceptible to postoperative infection.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalInternational Surgery
Volume90
Issue number5
Publication statusPublished - 2005

Fingerprint

Fertility
Swine
Autologous Transplantation
Blood Vessels
Thrombosis
Perfusion
Pelvic Infection
Transplants
Uterine Artery
Estrus
Ovariectomy
Hysterectomy
Transplantation
Pregnancy
Infection

Keywords

  • Infertility
  • Porcine model
  • Uterine transplantation

ASJC Scopus subject areas

  • Surgery

Cite this

Sieunarine, K., Zakaria, F. B. P., Boyle, D. C. M., Corless, D. J., Noakes, D. E., Lindsay, I., ... Smith, J. R. (2005). Possibilities for fertility restoration: A new surgical technique. International Surgery, 90(5), 249-256.

Possibilities for fertility restoration : A new surgical technique. / Sieunarine, Krishen; Zakaria, Faris B P; Boyle, Deborah C M; Corless, David J.; Noakes, David E.; Lindsay, Iain; Lawson, Andrew; Ungár, L.; Del Priore, Giuseppe; Smith, J. Richard.

In: International Surgery, Vol. 90, No. 5, 2005, p. 249-256.

Research output: Contribution to journalArticle

Sieunarine, K, Zakaria, FBP, Boyle, DCM, Corless, DJ, Noakes, DE, Lindsay, I, Lawson, A, Ungár, L, Del Priore, G & Smith, JR 2005, 'Possibilities for fertility restoration: A new surgical technique', International Surgery, vol. 90, no. 5, pp. 249-256.
Sieunarine K, Zakaria FBP, Boyle DCM, Corless DJ, Noakes DE, Lindsay I et al. Possibilities for fertility restoration: A new surgical technique. International Surgery. 2005;90(5):249-256.
Sieunarine, Krishen ; Zakaria, Faris B P ; Boyle, Deborah C M ; Corless, David J. ; Noakes, David E. ; Lindsay, Iain ; Lawson, Andrew ; Ungár, L. ; Del Priore, Giuseppe ; Smith, J. Richard. / Possibilities for fertility restoration : A new surgical technique. In: International Surgery. 2005 ; Vol. 90, No. 5. pp. 249-256.
@article{38ef1ee0cd1f4f9fafeca2ad10a5cbbb,
title = "Possibilities for fertility restoration: A new surgical technique",
abstract = "Previously published work concluded that uterine artery microvascular anastomosis in the porcine model was feasible with subsequent normal vascular function in pregnancy of the anastomosed vessels. The objective of this study was to assess the feasibility of uterine autotransplantation in a porcine model using microvascular anastomoses. Eight large white/landrace sows of proven fertility were used. A supracervical hysterectomy with or without bilateral salpingo-oophorectomy (BSO) was performed. After 1 hour of cold storage in a transplant solution, the specimen was reintroduced and followed by stepwise vascular reanastomosis. Objective perfusion index measurements suggested adequate uterine perfusion after transplantation. Postoperatively, sow 1 survived to 3 months with no signs of oestrus, and sows 2, 6, and 8 were killed on days 6, 33, and 54, respectively, for pelvic infection. Histopathology of the uterine grafts revealed gradual vessel thromboses. Microvascular reanastomosis is feasible, however, unsuccessful in uterine autotransplantation because of gradual vessel thromboses. The porcine model is highly susceptible to postoperative infection.",
keywords = "Infertility, Porcine model, Uterine transplantation",
author = "Krishen Sieunarine and Zakaria, {Faris B P} and Boyle, {Deborah C M} and Corless, {David J.} and Noakes, {David E.} and Iain Lindsay and Andrew Lawson and L. Ung{\'a}r and {Del Priore}, Giuseppe and Smith, {J. Richard}",
year = "2005",
language = "English",
volume = "90",
pages = "249--256",
journal = "International Surgery",
issn = "0020-8868",
publisher = "International College of Surgeons",
number = "5",

}

TY - JOUR

T1 - Possibilities for fertility restoration

T2 - A new surgical technique

AU - Sieunarine, Krishen

AU - Zakaria, Faris B P

AU - Boyle, Deborah C M

AU - Corless, David J.

AU - Noakes, David E.

AU - Lindsay, Iain

AU - Lawson, Andrew

AU - Ungár, L.

AU - Del Priore, Giuseppe

AU - Smith, J. Richard

PY - 2005

Y1 - 2005

N2 - Previously published work concluded that uterine artery microvascular anastomosis in the porcine model was feasible with subsequent normal vascular function in pregnancy of the anastomosed vessels. The objective of this study was to assess the feasibility of uterine autotransplantation in a porcine model using microvascular anastomoses. Eight large white/landrace sows of proven fertility were used. A supracervical hysterectomy with or without bilateral salpingo-oophorectomy (BSO) was performed. After 1 hour of cold storage in a transplant solution, the specimen was reintroduced and followed by stepwise vascular reanastomosis. Objective perfusion index measurements suggested adequate uterine perfusion after transplantation. Postoperatively, sow 1 survived to 3 months with no signs of oestrus, and sows 2, 6, and 8 were killed on days 6, 33, and 54, respectively, for pelvic infection. Histopathology of the uterine grafts revealed gradual vessel thromboses. Microvascular reanastomosis is feasible, however, unsuccessful in uterine autotransplantation because of gradual vessel thromboses. The porcine model is highly susceptible to postoperative infection.

AB - Previously published work concluded that uterine artery microvascular anastomosis in the porcine model was feasible with subsequent normal vascular function in pregnancy of the anastomosed vessels. The objective of this study was to assess the feasibility of uterine autotransplantation in a porcine model using microvascular anastomoses. Eight large white/landrace sows of proven fertility were used. A supracervical hysterectomy with or without bilateral salpingo-oophorectomy (BSO) was performed. After 1 hour of cold storage in a transplant solution, the specimen was reintroduced and followed by stepwise vascular reanastomosis. Objective perfusion index measurements suggested adequate uterine perfusion after transplantation. Postoperatively, sow 1 survived to 3 months with no signs of oestrus, and sows 2, 6, and 8 were killed on days 6, 33, and 54, respectively, for pelvic infection. Histopathology of the uterine grafts revealed gradual vessel thromboses. Microvascular reanastomosis is feasible, however, unsuccessful in uterine autotransplantation because of gradual vessel thromboses. The porcine model is highly susceptible to postoperative infection.

KW - Infertility

KW - Porcine model

KW - Uterine transplantation

UR - http://www.scopus.com/inward/record.url?scp=33747117402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747117402&partnerID=8YFLogxK

M3 - Article

VL - 90

SP - 249

EP - 256

JO - International Surgery

JF - International Surgery

SN - 0020-8868

IS - 5

ER -