Positive inotropic effect of the inhibition of cyclic GMP-stimulated 3′,5′-cyclic nucleotide phosphodiesterase (PDE2) on guinea pig left atria in Eu- and hyperthyroidism

R. Gesztelyi, J. Zsuga, P. Hajdú, J. Szabó, A. Cseppento, A. J. Szentmiklósi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The significance of PDE2 on the atrial inotropy was studied in euand hyperthyroidism. The contractile force was measured and negative inotropic capacity of N6-cyclopentyladenosine (CPA) was determined on left atria isolated from 8-day thyroxine- or solvent-treated guinea pigs, in the presence or absence of EHNA (adenosine deaminase and PDE2 inhibitor) or NBTI (nucleoside transporter inhibitor). EHNA was administered to inhibit PDE2, while NBTI was used to model the accumulation of endogenous adenosine. The reduction of the contractile force caused by EHNA was smaller in the thyroxine-treated atria than in the solvent-treated samples. Contrary, NBTI induced a decrease in the contractile force without significant difference between the two groups. In addition, EHNA enhanced the efficiency of CPA in thyroxine-treated atria and did not affect it in solvent-treated samples, while the response to CPA was decreased by NBTI in all atria, especially in hyperthyroidism. On the basis of greater retention of the contractile force and sustained/enhanced responsiveness to CPA in the presence of EHNA we conclude that PDE2's inhibition has a significant positive inotropic effect in guinea pig atria and this effect is proven to be augmented in hyperthyroidism.

Original languageEnglish
Pages (from-to)501-513
Number of pages13
JournalGeneral Physiology and Biophysics
Volume22
Issue number4
Publication statusPublished - Dec 2003

Fingerprint

Type 2 Cyclic Nucleotide Phosphodiesterases
Cyclic Nucleotides
Cyclic GMP
Hyperthyroidism
Heart Atria
Guinea Pigs
Thyroxine
Adenosine Deaminase Inhibitors
Nucleoside Transport Proteins
Adenosine
9-(2-hydroxy-3-nonyl)adenine
4-nitrobenzylthioinosine

Keywords

  • Adenosine
  • Guinea pigs
  • Inotropic effect
  • PDE
  • Thyroxine

ASJC Scopus subject areas

  • Physiology
  • Biophysics

Cite this

Positive inotropic effect of the inhibition of cyclic GMP-stimulated 3′,5′-cyclic nucleotide phosphodiesterase (PDE2) on guinea pig left atria in Eu- and hyperthyroidism. / Gesztelyi, R.; Zsuga, J.; Hajdú, P.; Szabó, J.; Cseppento, A.; Szentmiklósi, A. J.

In: General Physiology and Biophysics, Vol. 22, No. 4, 12.2003, p. 501-513.

Research output: Contribution to journalArticle

@article{01a42612e3694861b434632d2476466b,
title = "Positive inotropic effect of the inhibition of cyclic GMP-stimulated 3′,5′-cyclic nucleotide phosphodiesterase (PDE2) on guinea pig left atria in Eu- and hyperthyroidism",
abstract = "The significance of PDE2 on the atrial inotropy was studied in euand hyperthyroidism. The contractile force was measured and negative inotropic capacity of N6-cyclopentyladenosine (CPA) was determined on left atria isolated from 8-day thyroxine- or solvent-treated guinea pigs, in the presence or absence of EHNA (adenosine deaminase and PDE2 inhibitor) or NBTI (nucleoside transporter inhibitor). EHNA was administered to inhibit PDE2, while NBTI was used to model the accumulation of endogenous adenosine. The reduction of the contractile force caused by EHNA was smaller in the thyroxine-treated atria than in the solvent-treated samples. Contrary, NBTI induced a decrease in the contractile force without significant difference between the two groups. In addition, EHNA enhanced the efficiency of CPA in thyroxine-treated atria and did not affect it in solvent-treated samples, while the response to CPA was decreased by NBTI in all atria, especially in hyperthyroidism. On the basis of greater retention of the contractile force and sustained/enhanced responsiveness to CPA in the presence of EHNA we conclude that PDE2's inhibition has a significant positive inotropic effect in guinea pig atria and this effect is proven to be augmented in hyperthyroidism.",
keywords = "Adenosine, Guinea pigs, Inotropic effect, PDE, Thyroxine",
author = "R. Gesztelyi and J. Zsuga and P. Hajd{\'u} and J. Szab{\'o} and A. Cseppento and Szentmikl{\'o}si, {A. J.}",
year = "2003",
month = "12",
language = "English",
volume = "22",
pages = "501--513",
journal = "General Physiology and Biophysics",
issn = "0231-5882",
publisher = "Slovak Academy of Sciences",
number = "4",

}

TY - JOUR

T1 - Positive inotropic effect of the inhibition of cyclic GMP-stimulated 3′,5′-cyclic nucleotide phosphodiesterase (PDE2) on guinea pig left atria in Eu- and hyperthyroidism

AU - Gesztelyi, R.

AU - Zsuga, J.

AU - Hajdú, P.

AU - Szabó, J.

AU - Cseppento, A.

AU - Szentmiklósi, A. J.

PY - 2003/12

Y1 - 2003/12

N2 - The significance of PDE2 on the atrial inotropy was studied in euand hyperthyroidism. The contractile force was measured and negative inotropic capacity of N6-cyclopentyladenosine (CPA) was determined on left atria isolated from 8-day thyroxine- or solvent-treated guinea pigs, in the presence or absence of EHNA (adenosine deaminase and PDE2 inhibitor) or NBTI (nucleoside transporter inhibitor). EHNA was administered to inhibit PDE2, while NBTI was used to model the accumulation of endogenous adenosine. The reduction of the contractile force caused by EHNA was smaller in the thyroxine-treated atria than in the solvent-treated samples. Contrary, NBTI induced a decrease in the contractile force without significant difference between the two groups. In addition, EHNA enhanced the efficiency of CPA in thyroxine-treated atria and did not affect it in solvent-treated samples, while the response to CPA was decreased by NBTI in all atria, especially in hyperthyroidism. On the basis of greater retention of the contractile force and sustained/enhanced responsiveness to CPA in the presence of EHNA we conclude that PDE2's inhibition has a significant positive inotropic effect in guinea pig atria and this effect is proven to be augmented in hyperthyroidism.

AB - The significance of PDE2 on the atrial inotropy was studied in euand hyperthyroidism. The contractile force was measured and negative inotropic capacity of N6-cyclopentyladenosine (CPA) was determined on left atria isolated from 8-day thyroxine- or solvent-treated guinea pigs, in the presence or absence of EHNA (adenosine deaminase and PDE2 inhibitor) or NBTI (nucleoside transporter inhibitor). EHNA was administered to inhibit PDE2, while NBTI was used to model the accumulation of endogenous adenosine. The reduction of the contractile force caused by EHNA was smaller in the thyroxine-treated atria than in the solvent-treated samples. Contrary, NBTI induced a decrease in the contractile force without significant difference between the two groups. In addition, EHNA enhanced the efficiency of CPA in thyroxine-treated atria and did not affect it in solvent-treated samples, while the response to CPA was decreased by NBTI in all atria, especially in hyperthyroidism. On the basis of greater retention of the contractile force and sustained/enhanced responsiveness to CPA in the presence of EHNA we conclude that PDE2's inhibition has a significant positive inotropic effect in guinea pig atria and this effect is proven to be augmented in hyperthyroidism.

KW - Adenosine

KW - Guinea pigs

KW - Inotropic effect

KW - PDE

KW - Thyroxine

UR - http://www.scopus.com/inward/record.url?scp=1542346363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542346363&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 501

EP - 513

JO - General Physiology and Biophysics

JF - General Physiology and Biophysics

SN - 0231-5882

IS - 4

ER -