Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation

É. Kondorosi, J. Gyuris, J. Schmidt, M. John, E. Duda, B. Hoffmann, J. Schell, A. Kondorosi

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We show that expression of common nodulation genes in Rhizobium meliloti is under positive as well as negative control. A repressor protein was found to be involved in the negative control of nod gene expression. Whereas the activator NodD protein binds to the conserved cis-regulatory element (nod-box) required for coordinated regulation of nod genes, the repressor binds to the overlapping nodD1 and nodA promoters, at the RNA polymerase binding site. A model depicting the possible interaction of the plant-derived nod gene inducer (luteolin), the NodD and the repressor with the nod promoter elements is presented. Mutants lacking the repressor exhibited delayed nodulation phenotype, indicating that fine tuning of nod gene expression is required for optimal nodulation of the plant host.

Original languageEnglish
Pages (from-to)1331-1340
Number of pages10
JournalEMBO Journal
Volume8
Issue number5
Publication statusPublished - 1989

Fingerprint

Sinorhizobium meliloti
Gene expression
Genes
Gene Expression
Repressor Proteins
Luteolin
DNA-Directed RNA Polymerases
Tuning
Binding Sites
Phenotype
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Kondorosi, É., Gyuris, J., Schmidt, J., John, M., Duda, E., Hoffmann, B., ... Kondorosi, A. (1989). Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation. EMBO Journal, 8(5), 1331-1340.

Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation. / Kondorosi, É.; Gyuris, J.; Schmidt, J.; John, M.; Duda, E.; Hoffmann, B.; Schell, J.; Kondorosi, A.

In: EMBO Journal, Vol. 8, No. 5, 1989, p. 1331-1340.

Research output: Contribution to journalArticle

Kondorosi, É, Gyuris, J, Schmidt, J, John, M, Duda, E, Hoffmann, B, Schell, J & Kondorosi, A 1989, 'Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation', EMBO Journal, vol. 8, no. 5, pp. 1331-1340.
Kondorosi, É. ; Gyuris, J. ; Schmidt, J. ; John, M. ; Duda, E. ; Hoffmann, B. ; Schell, J. ; Kondorosi, A. / Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation. In: EMBO Journal. 1989 ; Vol. 8, No. 5. pp. 1331-1340.
@article{be2a8559961e4b2ea5ee807116b99747,
title = "Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation",
abstract = "We show that expression of common nodulation genes in Rhizobium meliloti is under positive as well as negative control. A repressor protein was found to be involved in the negative control of nod gene expression. Whereas the activator NodD protein binds to the conserved cis-regulatory element (nod-box) required for coordinated regulation of nod genes, the repressor binds to the overlapping nodD1 and nodA promoters, at the RNA polymerase binding site. A model depicting the possible interaction of the plant-derived nod gene inducer (luteolin), the NodD and the repressor with the nod promoter elements is presented. Mutants lacking the repressor exhibited delayed nodulation phenotype, indicating that fine tuning of nod gene expression is required for optimal nodulation of the plant host.",
author = "{\'E}. Kondorosi and J. Gyuris and J. Schmidt and M. John and E. Duda and B. Hoffmann and J. Schell and A. Kondorosi",
year = "1989",
language = "English",
volume = "8",
pages = "1331--1340",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation

AU - Kondorosi, É.

AU - Gyuris, J.

AU - Schmidt, J.

AU - John, M.

AU - Duda, E.

AU - Hoffmann, B.

AU - Schell, J.

AU - Kondorosi, A.

PY - 1989

Y1 - 1989

N2 - We show that expression of common nodulation genes in Rhizobium meliloti is under positive as well as negative control. A repressor protein was found to be involved in the negative control of nod gene expression. Whereas the activator NodD protein binds to the conserved cis-regulatory element (nod-box) required for coordinated regulation of nod genes, the repressor binds to the overlapping nodD1 and nodA promoters, at the RNA polymerase binding site. A model depicting the possible interaction of the plant-derived nod gene inducer (luteolin), the NodD and the repressor with the nod promoter elements is presented. Mutants lacking the repressor exhibited delayed nodulation phenotype, indicating that fine tuning of nod gene expression is required for optimal nodulation of the plant host.

AB - We show that expression of common nodulation genes in Rhizobium meliloti is under positive as well as negative control. A repressor protein was found to be involved in the negative control of nod gene expression. Whereas the activator NodD protein binds to the conserved cis-regulatory element (nod-box) required for coordinated regulation of nod genes, the repressor binds to the overlapping nodD1 and nodA promoters, at the RNA polymerase binding site. A model depicting the possible interaction of the plant-derived nod gene inducer (luteolin), the NodD and the repressor with the nod promoter elements is presented. Mutants lacking the repressor exhibited delayed nodulation phenotype, indicating that fine tuning of nod gene expression is required for optimal nodulation of the plant host.

UR - http://www.scopus.com/inward/record.url?scp=0024384879&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024384879&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 1331

EP - 1340

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 5

ER -