Populations of hippocampal inhibitory neurons express different levels of cytochrome c

Attila I. Gulyás, György Buzsáki, T. Freund, Hajime Hirase

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Cytochrome c (CC) immunoreactivity was quantified in functionally distinct rat hippocampal inhibitory neuron populations using double immunocytochemistry and laser scanning confocal microscopy to measure the CC expression level as well as the amount of mitochondria within the cells, which is a sign of neuronal activity. The CC signal showed a similar distribution to cytochrome c oxidase histochemical staining. Strongly stained somata, dendrites and axon terminal clouds were dispersed over the low intensity neuropil staining. The staining was granular and electron microscopic investigation confirmed that the signal was localized in mitochondria. Intensively labeled neurons, showing the morphological features of inhibitory cells, were most frequently found in the principal cell layers, stratum oriens of the CA1-3 areas, stratum lucidum and hilus. These neurons contained not only a higher number of mitochondria than the principal cells but the intensity of the mitochondrial staining was evidently stronger. Among the examined interneuronal populations, parvalbumin- immunoreactive neurons were intensively labeled for CC. Calbindin D28k- (CB), somatostatin- and cholecystokinin-labeled cells showed heterogeneous CC levels, whereas calretinin-immunoreactive cells never showed a strong CC signal. CB cells in stratum oriens and alveus layers, lucidum and the hilus were strongly labeled for CC. CB cells in such regions are known to project to the medial septum and contain somatostatin. We have demonstrated that the CA1 interneurons that project to the medial septum (hippocampo-septal neurons) express a high level of CC. Thus, similar to the parvalbumin-containing basket and axo-axonic cells, the hippocampo-septal neurons potentially have a high average activity level.

Original languageEnglish
Pages (from-to)2581-2594
Number of pages14
JournalEuropean Journal of Neuroscience
Volume23
Issue number10
DOIs
Publication statusPublished - May 2006

Fingerprint

Cytochromes c
Neurons
Calbindin 1
Population
Staining and Labeling
Parvalbumins
Mitochondria
Somatostatin
Calbindin 2
Neuropil
Cholecystokinin
Presynaptic Terminals
Carisoprodol
Interneurons
Electron Transport Complex IV
Dendrites
Confocal Microscopy
Immunohistochemistry
Electrons

Keywords

  • Basket cells
  • GABAergic neurons
  • Medial septum
  • Neurochemical markers
  • Oriens/lacunosum-moleculare cells
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Populations of hippocampal inhibitory neurons express different levels of cytochrome c. / Gulyás, Attila I.; Buzsáki, György; Freund, T.; Hirase, Hajime.

In: European Journal of Neuroscience, Vol. 23, No. 10, 05.2006, p. 2581-2594.

Research output: Contribution to journalArticle

Gulyás, Attila I. ; Buzsáki, György ; Freund, T. ; Hirase, Hajime. / Populations of hippocampal inhibitory neurons express different levels of cytochrome c. In: European Journal of Neuroscience. 2006 ; Vol. 23, No. 10. pp. 2581-2594.
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