Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice

Istvan Sebe, Eszter Ostorhazi, Aron Fekete, Krisztian N. Kovacs, R. Zelkó, I. Kovalszky, Wenyi Li, John D. Wade, D. Szabó, Laszlo Otvos

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Native and designer cationic antimicrobial peptides are increasingly acknowledged as host defense molecules rather than true antimicrobials. Due to their ability to activate the innate immune system, these structures are used to treat uninfected and bacterially-infected wounds, including those harboring Acinetobacter baumannii. Previously we documented that when administered intramuscularly or topically in liquid formulations, the proline-rich host defense peptide dimer A3-APO accelerates uninfected wound re-epithelization and eliminates systemic and local A. baumannii, methicillin-resistant Staphylococcus aureus and other pathogen load from infected lesions better than conventional antibiotics. In the current study we sought to produce and characterize a novel delivery system, suitable for immediate and convenient application in non-hospital environments. The APO monomer was incorporated into polyvinyl alcohol nanofibers and the complex was polymerized into a solid patch dressing. Mice were subjected to skin abrasion where the wounds were either left uninfected or were inoculated with a near lethal dose of multidrug resistant A. baumannii strain. Analyzed after 3 days, APO monomer-containing patches improved wound appearance significantly better than polymer patches without antibiotics. When compared to colistin, the APO patches accelerated wound healing, and statistically significantly reduced wound size and wound bacterial load. The in vivo antimicrobial effect was more extensive than after intramuscular administration of the peptide drug, by using only one tenth of the active pharmaceutical ingredient. These data suggest that the APO monomer-impregnated nanofiber dressing can be developed as an economical first-line treatment option to skin injuries in general and battlefield burn and blast injuries in particular.

Original languageEnglish
JournalAmino Acids
DOIs
Publication statusAccepted/In press - Aug 29 2015

Fingerprint

Nanofibers
Polyvinyl Alcohol
Acinetobacter baumannii
Skin
Monomers
Peptides
Wounds and Injuries
Antimicrobial Cationic Peptides
Anti-Bacterial Agents
Colistin
Methicillin
Immune system
Pathogens
Abrasion
Proline
Pharmaceutical Preparations
Dimers
Bandages
Polymers
Blast Injuries

Keywords

  • Antibacterial peptide
  • Cutaneous infection
  • Nanoformulation
  • Topical treatment
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Polyvinyl alcohol nanofiber formulation of the designer antimicrobial peptide APO sterilizes Acinetobacter baumannii-infected skin wounds in mice. / Sebe, Istvan; Ostorhazi, Eszter; Fekete, Aron; Kovacs, Krisztian N.; Zelkó, R.; Kovalszky, I.; Li, Wenyi; Wade, John D.; Szabó, D.; Otvos, Laszlo.

In: Amino Acids, 29.08.2015.

Research output: Contribution to journalArticle

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