Polyploidy in the haplontic yeast Schizosaccharomyces pombe: construction and analysis of strains

Research output: Contribution to journalArticle

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Abstract

The fission yeast Schizosaccharomyces pombe has a haplontic life cycle in which the diplophase is confined to the zygote. Through the use of one- and two-step protoplast fusions we show that the ploidy can be increased up to pentaploid. The polyploid fusion products are rather unstable and segregate cells of lower ploidies by gradual loss of chromosomes during mitotic divisions. The polyploid cells conjugate normally but are prone to arrest at various stages of meiosis (1-, 2- and 3-spored asci, binucleate spores) and/or produce inviable, most probably aneuploid, spores. Marker segregation in the complete tetrads indicates the multiple association of homologous chromosomes. In tetra- and penta-ploid meiosis, multispored (6- to 7-spored) asci are also produced, probably by postmeiotic division of the nuclei.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalCurrent Genetics
Volume24
Issue number1-2
DOIs
Publication statusPublished - Jul 1993

Fingerprint

Polyploidy
Schizosaccharomyces
Ploidies
Meiosis
Spores
Chromosomes
Yeasts
Rubiaceae
Protoplasts
Zygote
Aneuploidy
Life Cycle Stages

Keywords

  • Fission yeast
  • Meiosis
  • Mitotic stability
  • Polyploidy

ASJC Scopus subject areas

  • Genetics

Cite this

Polyploidy in the haplontic yeast Schizosaccharomyces pombe : construction and analysis of strains. / Molnár, M.; Sipiczki, M.

In: Current Genetics, Vol. 24, No. 1-2, 07.1993, p. 45-52.

Research output: Contribution to journalArticle

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