Polymorphisms of the ApoE, HSD3B1, IL-1β and p53 genes are associated with the development of early uremic complications in diabetic patients: Results of a DNA resequencing array study

Dominika Szoke, B. Molnár, N. Solymosi, K. Rácz, Peter Gergics, B. Blaskó, B. Vásárhelyi, A. Vannay, Yvette Mandy, Gergely Klausz, Zsofia Gyulai, O. Galamb, S. Spisák, Bernadett Hutkai, A. Somogyi, Klara Berta, Andras Szabo, T. Tulassay, Z. Tulassay

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Abstract

Genetic polymorphisms of the genes involved in angiogenesis, the inflammatory cascade or apoptosis control can influence the chronic complications of diabetic patients. Parallel evaluation of multiple genetic polymorphisms became available with the development of DNA resequencing arrays. We aimed to develop a 16-gene, 18,859-nucleotide resequencing array to analyze the genetic background of uremic and gastrointestinal complications. DNA was isolated from 10 ml of peripheral blood of 41 non-uremic and 37 uremic patients with type II diabetes mellitus (DM); 32 suffering from gastric erosion complications. An Affymetrix Customseq Resequencing array was developed containing a total of 37 PCR products of selected genes. Confirmatory analysis was performed for 5 known polymorphisms by RFLP and for 4 others by capillary sequencing. Statistical analysis was performed using the Fisher's exact test. Correlations between the DNA resequencing array and the confirmatory methods were 96% for RFLP and 99.4% for capillary sequencing. The genetic polymorphisms of the ApoE, HSD3B1, IL-1β and p53 genes were found to be significantly different (p

Original languageEnglish
Pages (from-to)217-227
Number of pages11
JournalInternational Journal of Molecular Medicine
Volume23
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

p53 Genes
Apolipoproteins E
Genetic Polymorphisms
Diabetes Complications
Oligonucleotide Array Sequence Analysis
Interleukin-1
Restriction Fragment Length Polymorphisms
Genes
Psychological Stress
Type 2 Diabetes Mellitus
Stomach
Nucleotides
Apoptosis
Polymerase Chain Reaction
DNA

Keywords

  • p53 polymorphism
  • Resequencing array
  • Type II diabetes mellitus
  • Uremia

ASJC Scopus subject areas

  • Genetics

Cite this

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title = "Polymorphisms of the ApoE, HSD3B1, IL-1β and p53 genes are associated with the development of early uremic complications in diabetic patients: Results of a DNA resequencing array study",
abstract = "Genetic polymorphisms of the genes involved in angiogenesis, the inflammatory cascade or apoptosis control can influence the chronic complications of diabetic patients. Parallel evaluation of multiple genetic polymorphisms became available with the development of DNA resequencing arrays. We aimed to develop a 16-gene, 18,859-nucleotide resequencing array to analyze the genetic background of uremic and gastrointestinal complications. DNA was isolated from 10 ml of peripheral blood of 41 non-uremic and 37 uremic patients with type II diabetes mellitus (DM); 32 suffering from gastric erosion complications. An Affymetrix Customseq Resequencing array was developed containing a total of 37 PCR products of selected genes. Confirmatory analysis was performed for 5 known polymorphisms by RFLP and for 4 others by capillary sequencing. Statistical analysis was performed using the Fisher's exact test. Correlations between the DNA resequencing array and the confirmatory methods were 96{\%} for RFLP and 99.4{\%} for capillary sequencing. The genetic polymorphisms of the ApoE, HSD3B1, IL-1β and p53 genes were found to be significantly different (p",
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author = "Dominika Szoke and B. Moln{\'a}r and N. Solymosi and K. R{\'a}cz and Peter Gergics and B. Blask{\'o} and B. V{\'a}s{\'a}rhelyi and A. Vannay and Yvette Mandy and Gergely Klausz and Zsofia Gyulai and O. Galamb and S. Spis{\'a}k and Bernadett Hutkai and A. Somogyi and Klara Berta and Andras Szabo and T. Tulassay and Z. Tulassay",
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T1 - Polymorphisms of the ApoE, HSD3B1, IL-1β and p53 genes are associated with the development of early uremic complications in diabetic patients

T2 - Results of a DNA resequencing array study

AU - Szoke, Dominika

AU - Molnár, B.

AU - Solymosi, N.

AU - Rácz, K.

AU - Gergics, Peter

AU - Blaskó, B.

AU - Vásárhelyi, B.

AU - Vannay, A.

AU - Mandy, Yvette

AU - Klausz, Gergely

AU - Gyulai, Zsofia

AU - Galamb, O.

AU - Spisák, S.

AU - Hutkai, Bernadett

AU - Somogyi, A.

AU - Berta, Klara

AU - Szabo, Andras

AU - Tulassay, T.

AU - Tulassay, Z.

PY - 2009

Y1 - 2009

N2 - Genetic polymorphisms of the genes involved in angiogenesis, the inflammatory cascade or apoptosis control can influence the chronic complications of diabetic patients. Parallel evaluation of multiple genetic polymorphisms became available with the development of DNA resequencing arrays. We aimed to develop a 16-gene, 18,859-nucleotide resequencing array to analyze the genetic background of uremic and gastrointestinal complications. DNA was isolated from 10 ml of peripheral blood of 41 non-uremic and 37 uremic patients with type II diabetes mellitus (DM); 32 suffering from gastric erosion complications. An Affymetrix Customseq Resequencing array was developed containing a total of 37 PCR products of selected genes. Confirmatory analysis was performed for 5 known polymorphisms by RFLP and for 4 others by capillary sequencing. Statistical analysis was performed using the Fisher's exact test. Correlations between the DNA resequencing array and the confirmatory methods were 96% for RFLP and 99.4% for capillary sequencing. The genetic polymorphisms of the ApoE, HSD3B1, IL-1β and p53 genes were found to be significantly different (p

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