Polymorphism of the heat-shock protein gene Hsp70-2, but not polymorphisms of the IL-10 and CD14 genes, is associated with the outcome of Crohn's disease

Gergely Klausz, T. Molnár, F. Nagy, Zsófia Gyulai, K. Boda, J. Lonovics, Y. Mándi

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41 Citations (Scopus)

Abstract

Objective. In Crohn's disease (CD) a Th-1 dominant immune reaction is induced, which could be associated with genetic predisposition. Several previous studies have investigated the roles of CD14, heat-shock protein (Hsp)70 and IL-10 gene polymorphisms in the development of the disease. The results are contradictory and inter-racial differences are implicated. Therefore, this phenomenon was evaluated in well-documented Caucasian patients with CD in order to verify the clinical importance of these polymorphisms. Material and methods. The genomic DNA of 133 patients with CD and that of 75 healthy controls were examined. CD was divided into subgroups according to the Vienna classification. An arbitrary classification system based on disease severity was also applied, which was determined according to the therapeutic intervention. The CD14 (- 159 C → T) promoter gene polymorphism was investigated by melting-point analysis. The IL-10 (- 1082 G → A) and Hsp70-2 (1267 A → G) gene polymorphisms were detected by RFLP (restriction fragment length polymorphism). Results. None of the allele frequencies of the examined polymorphisms differed significantly between the patient and control populations. Neither the CD14 nor the IL-10 polymorphisms exhibited any correlation with the development or with the progression of the disease. With regard to Hsp70-2 gene polymorphism, those patients who carry at least one A allele have a significantly lower probability of the need for surgical intervention. Conclusions. Allele A of the Hsp70-2 gene may be associated with a less severe form of CD, suggesting the clinical value of the genotype assessment. The genetic determination of the defense mechanisms in CD appears to be associated with the polymorphism of the Hsp70-2 gene rather than that of the CD14 or IL-10 genes.

Original languageEnglish
Pages (from-to)1197-1204
Number of pages8
JournalScandinavian Journal of Gastroenterology
Volume40
Issue number10
DOIs
Publication statusPublished - Oct 2005

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Heat-Shock Proteins
Crohn Disease
Interleukin-10
Genes
Alleles
HSP70 Heat-Shock Proteins
Genetic Predisposition to Disease
Gene Frequency
Restriction Fragment Length Polymorphisms
Freezing
Disease Progression
Genotype
DNA
Population

Keywords

  • CD14
  • Crohn's disease
  • Hsp70
  • IL-10
  • SNP

ASJC Scopus subject areas

  • Gastroenterology

Cite this

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title = "Polymorphism of the heat-shock protein gene Hsp70-2, but not polymorphisms of the IL-10 and CD14 genes, is associated with the outcome of Crohn's disease",
abstract = "Objective. In Crohn's disease (CD) a Th-1 dominant immune reaction is induced, which could be associated with genetic predisposition. Several previous studies have investigated the roles of CD14, heat-shock protein (Hsp)70 and IL-10 gene polymorphisms in the development of the disease. The results are contradictory and inter-racial differences are implicated. Therefore, this phenomenon was evaluated in well-documented Caucasian patients with CD in order to verify the clinical importance of these polymorphisms. Material and methods. The genomic DNA of 133 patients with CD and that of 75 healthy controls were examined. CD was divided into subgroups according to the Vienna classification. An arbitrary classification system based on disease severity was also applied, which was determined according to the therapeutic intervention. The CD14 (- 159 C → T) promoter gene polymorphism was investigated by melting-point analysis. The IL-10 (- 1082 G → A) and Hsp70-2 (1267 A → G) gene polymorphisms were detected by RFLP (restriction fragment length polymorphism). Results. None of the allele frequencies of the examined polymorphisms differed significantly between the patient and control populations. Neither the CD14 nor the IL-10 polymorphisms exhibited any correlation with the development or with the progression of the disease. With regard to Hsp70-2 gene polymorphism, those patients who carry at least one A allele have a significantly lower probability of the need for surgical intervention. Conclusions. Allele A of the Hsp70-2 gene may be associated with a less severe form of CD, suggesting the clinical value of the genotype assessment. The genetic determination of the defense mechanisms in CD appears to be associated with the polymorphism of the Hsp70-2 gene rather than that of the CD14 or IL-10 genes.",
keywords = "CD14, Crohn's disease, Hsp70, IL-10, SNP",
author = "Gergely Klausz and T. Moln{\'a}r and F. Nagy and Zs{\'o}fia Gyulai and K. Boda and J. Lonovics and Y. M{\'a}ndi",
year = "2005",
month = "10",
doi = "10.1080/00365520510023350",
language = "English",
volume = "40",
pages = "1197--1204",
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}

TY - JOUR

T1 - Polymorphism of the heat-shock protein gene Hsp70-2, but not polymorphisms of the IL-10 and CD14 genes, is associated with the outcome of Crohn's disease

AU - Klausz, Gergely

AU - Molnár, T.

AU - Nagy, F.

AU - Gyulai, Zsófia

AU - Boda, K.

AU - Lonovics, J.

AU - Mándi, Y.

PY - 2005/10

Y1 - 2005/10

N2 - Objective. In Crohn's disease (CD) a Th-1 dominant immune reaction is induced, which could be associated with genetic predisposition. Several previous studies have investigated the roles of CD14, heat-shock protein (Hsp)70 and IL-10 gene polymorphisms in the development of the disease. The results are contradictory and inter-racial differences are implicated. Therefore, this phenomenon was evaluated in well-documented Caucasian patients with CD in order to verify the clinical importance of these polymorphisms. Material and methods. The genomic DNA of 133 patients with CD and that of 75 healthy controls were examined. CD was divided into subgroups according to the Vienna classification. An arbitrary classification system based on disease severity was also applied, which was determined according to the therapeutic intervention. The CD14 (- 159 C → T) promoter gene polymorphism was investigated by melting-point analysis. The IL-10 (- 1082 G → A) and Hsp70-2 (1267 A → G) gene polymorphisms were detected by RFLP (restriction fragment length polymorphism). Results. None of the allele frequencies of the examined polymorphisms differed significantly between the patient and control populations. Neither the CD14 nor the IL-10 polymorphisms exhibited any correlation with the development or with the progression of the disease. With regard to Hsp70-2 gene polymorphism, those patients who carry at least one A allele have a significantly lower probability of the need for surgical intervention. Conclusions. Allele A of the Hsp70-2 gene may be associated with a less severe form of CD, suggesting the clinical value of the genotype assessment. The genetic determination of the defense mechanisms in CD appears to be associated with the polymorphism of the Hsp70-2 gene rather than that of the CD14 or IL-10 genes.

AB - Objective. In Crohn's disease (CD) a Th-1 dominant immune reaction is induced, which could be associated with genetic predisposition. Several previous studies have investigated the roles of CD14, heat-shock protein (Hsp)70 and IL-10 gene polymorphisms in the development of the disease. The results are contradictory and inter-racial differences are implicated. Therefore, this phenomenon was evaluated in well-documented Caucasian patients with CD in order to verify the clinical importance of these polymorphisms. Material and methods. The genomic DNA of 133 patients with CD and that of 75 healthy controls were examined. CD was divided into subgroups according to the Vienna classification. An arbitrary classification system based on disease severity was also applied, which was determined according to the therapeutic intervention. The CD14 (- 159 C → T) promoter gene polymorphism was investigated by melting-point analysis. The IL-10 (- 1082 G → A) and Hsp70-2 (1267 A → G) gene polymorphisms were detected by RFLP (restriction fragment length polymorphism). Results. None of the allele frequencies of the examined polymorphisms differed significantly between the patient and control populations. Neither the CD14 nor the IL-10 polymorphisms exhibited any correlation with the development or with the progression of the disease. With regard to Hsp70-2 gene polymorphism, those patients who carry at least one A allele have a significantly lower probability of the need for surgical intervention. Conclusions. Allele A of the Hsp70-2 gene may be associated with a less severe form of CD, suggesting the clinical value of the genotype assessment. The genetic determination of the defense mechanisms in CD appears to be associated with the polymorphism of the Hsp70-2 gene rather than that of the CD14 or IL-10 genes.

KW - CD14

KW - Crohn's disease

KW - Hsp70

KW - IL-10

KW - SNP

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