Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin

Gabriella Siesto, Angela Capece, M. Sipiczki, Hajnalka Csoma, Patrizia Romano

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, wild Saccharomyces cerevisiae strains, isolated from spontaneously fermenting grapes of different varieties and origins, were submitted to genetic analysis using different molecular techniques, such as amplification of genes coding for cell wall proteins and containing minisatellite-like sequences, karyotyping, mtDNA-RFLP, and analysis of the δ region. The lowest discriminative power was obtained by minisatellites analysis, in particular the amplification of AGA1 genes. Karyotyping and mtDNA-RFLP analysis yielded the same differentiation among the strains, whereas the PCR amplification of δ sequences resulted the best method as it was fast and it showed a very high discriminative power. In any case, it has to be underlined that some strains, showing the same delta profiles, exhibited a different mtDNA restriction profile and electrophoretic karyotype, suggesting that more than one molecular marker is required for reliable strain discrimination. Although the techniques used revealed a different resolution power, they all revealed a genetic relationship among strains isolated from spontaneous fermentation of grapes of different origins. In fact, none of the typing methods was able to discriminate some strains isolated from different areas.

Original languageEnglish
Pages (from-to)661-668
Number of pages8
JournalAnnals of Microbiology
Volume63
Issue number2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Wine
Mitochondrial DNA
Saccharomyces cerevisiae
Karyotyping
Minisatellite Repeats
Gene Amplification
Vitis
Restriction Fragment Length Polymorphisms
Karyotype
Cell Wall
Fermentation
Polymerase Chain Reaction
Proteins

Keywords

  • Interdelta region
  • Karyotyping
  • Minisatellites
  • mtDNA-RFLP
  • Saccharomyces cerevisiae
  • Wine strains

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin. / Siesto, Gabriella; Capece, Angela; Sipiczki, M.; Csoma, Hajnalka; Romano, Patrizia.

In: Annals of Microbiology, Vol. 63, No. 2, 06.2013, p. 661-668.

Research output: Contribution to journalArticle

Siesto, Gabriella ; Capece, Angela ; Sipiczki, M. ; Csoma, Hajnalka ; Romano, Patrizia. / Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin. In: Annals of Microbiology. 2013 ; Vol. 63, No. 2. pp. 661-668.
@article{8220d82a8e0145809b71a4d684446c20,
title = "Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin",
abstract = "In this study, wild Saccharomyces cerevisiae strains, isolated from spontaneously fermenting grapes of different varieties and origins, were submitted to genetic analysis using different molecular techniques, such as amplification of genes coding for cell wall proteins and containing minisatellite-like sequences, karyotyping, mtDNA-RFLP, and analysis of the δ region. The lowest discriminative power was obtained by minisatellites analysis, in particular the amplification of AGA1 genes. Karyotyping and mtDNA-RFLP analysis yielded the same differentiation among the strains, whereas the PCR amplification of δ sequences resulted the best method as it was fast and it showed a very high discriminative power. In any case, it has to be underlined that some strains, showing the same delta profiles, exhibited a different mtDNA restriction profile and electrophoretic karyotype, suggesting that more than one molecular marker is required for reliable strain discrimination. Although the techniques used revealed a different resolution power, they all revealed a genetic relationship among strains isolated from spontaneous fermentation of grapes of different origins. In fact, none of the typing methods was able to discriminate some strains isolated from different areas.",
keywords = "Interdelta region, Karyotyping, Minisatellites, mtDNA-RFLP, Saccharomyces cerevisiae, Wine strains",
author = "Gabriella Siesto and Angela Capece and M. Sipiczki and Hajnalka Csoma and Patrizia Romano",
year = "2013",
month = "6",
doi = "10.1007/s13213-012-0516-6",
language = "English",
volume = "63",
pages = "661--668",
journal = "Annals of Microbiology",
issn = "1590-4261",
publisher = "Springer Heidelberg",
number = "2",

}

TY - JOUR

T1 - Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin

AU - Siesto, Gabriella

AU - Capece, Angela

AU - Sipiczki, M.

AU - Csoma, Hajnalka

AU - Romano, Patrizia

PY - 2013/6

Y1 - 2013/6

N2 - In this study, wild Saccharomyces cerevisiae strains, isolated from spontaneously fermenting grapes of different varieties and origins, were submitted to genetic analysis using different molecular techniques, such as amplification of genes coding for cell wall proteins and containing minisatellite-like sequences, karyotyping, mtDNA-RFLP, and analysis of the δ region. The lowest discriminative power was obtained by minisatellites analysis, in particular the amplification of AGA1 genes. Karyotyping and mtDNA-RFLP analysis yielded the same differentiation among the strains, whereas the PCR amplification of δ sequences resulted the best method as it was fast and it showed a very high discriminative power. In any case, it has to be underlined that some strains, showing the same delta profiles, exhibited a different mtDNA restriction profile and electrophoretic karyotype, suggesting that more than one molecular marker is required for reliable strain discrimination. Although the techniques used revealed a different resolution power, they all revealed a genetic relationship among strains isolated from spontaneous fermentation of grapes of different origins. In fact, none of the typing methods was able to discriminate some strains isolated from different areas.

AB - In this study, wild Saccharomyces cerevisiae strains, isolated from spontaneously fermenting grapes of different varieties and origins, were submitted to genetic analysis using different molecular techniques, such as amplification of genes coding for cell wall proteins and containing minisatellite-like sequences, karyotyping, mtDNA-RFLP, and analysis of the δ region. The lowest discriminative power was obtained by minisatellites analysis, in particular the amplification of AGA1 genes. Karyotyping and mtDNA-RFLP analysis yielded the same differentiation among the strains, whereas the PCR amplification of δ sequences resulted the best method as it was fast and it showed a very high discriminative power. In any case, it has to be underlined that some strains, showing the same delta profiles, exhibited a different mtDNA restriction profile and electrophoretic karyotype, suggesting that more than one molecular marker is required for reliable strain discrimination. Although the techniques used revealed a different resolution power, they all revealed a genetic relationship among strains isolated from spontaneous fermentation of grapes of different origins. In fact, none of the typing methods was able to discriminate some strains isolated from different areas.

KW - Interdelta region

KW - Karyotyping

KW - Minisatellites

KW - mtDNA-RFLP

KW - Saccharomyces cerevisiae

KW - Wine strains

UR - http://www.scopus.com/inward/record.url?scp=84878011489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878011489&partnerID=8YFLogxK

U2 - 10.1007/s13213-012-0516-6

DO - 10.1007/s13213-012-0516-6

M3 - Article

VL - 63

SP - 661

EP - 668

JO - Annals of Microbiology

JF - Annals of Microbiology

SN - 1590-4261

IS - 2

ER -