Polyethylene imine-based receptor immobilization for label free bioassays

S. Kurunczi, A. Hainard, K. Juhasz, D. Patko, N. Orgovan, N. Turck, J. C. Sanchez, R. Horváth

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume181
DOIs
Publication statusPublished - 2013

Fingerprint

bioassay
Imines
Bioassay
Polyethylene
immobilization
C-Reactive Protein
imines
Polyethylenes
Labels
polyethylenes
proteins
Proteins
antibodies
Antibodies
Assays
Immobilized Antibodies
biotin
Optical waveguides
Biotin
bioinstrumentation

Keywords

  • C-reactive protein (CRP)
  • Label free sensor
  • Neutravidin
  • OWLS
  • Polyethylene imine (PEI)
  • Room temperature immobilization

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Polyethylene imine-based receptor immobilization for label free bioassays. / Kurunczi, S.; Hainard, A.; Juhasz, K.; Patko, D.; Orgovan, N.; Turck, N.; Sanchez, J. C.; Horváth, R.

In: Sensors and Actuators, B: Chemical, Vol. 181, 2013, p. 71-76.

Research output: Contribution to journalArticle

Kurunczi, S, Hainard, A, Juhasz, K, Patko, D, Orgovan, N, Turck, N, Sanchez, JC & Horváth, R 2013, 'Polyethylene imine-based receptor immobilization for label free bioassays', Sensors and Actuators, B: Chemical, vol. 181, pp. 71-76. https://doi.org/10.1016/j.snb.2012.12.097
Kurunczi, S. ; Hainard, A. ; Juhasz, K. ; Patko, D. ; Orgovan, N. ; Turck, N. ; Sanchez, J. C. ; Horváth, R. / Polyethylene imine-based receptor immobilization for label free bioassays. In: Sensors and Actuators, B: Chemical. 2013 ; Vol. 181. pp. 71-76.
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