Polyethylene imine-based receptor immobilization for label free bioassays

S. Kurunczi, A. Hainard, K. Juhasz, D. Patko, N. Orgovan, N. Turck, J. C. Sanchez, R. Horvath

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume181
DOIs
Publication statusPublished - Mar 25 2013

Keywords

  • C-reactive protein (CRP)
  • Label free sensor
  • Neutravidin
  • OWLS
  • Polyethylene imine (PEI)
  • Room temperature immobilization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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  • Cite this

    Kurunczi, S., Hainard, A., Juhasz, K., Patko, D., Orgovan, N., Turck, N., Sanchez, J. C., & Horvath, R. (2013). Polyethylene imine-based receptor immobilization for label free bioassays. Sensors and Actuators, B: Chemical, 181, 71-76. https://doi.org/10.1016/j.snb.2012.12.097