Polyethylene glycol enhanced refolding of the recombinant human tissue transglutaminase

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7 Citations (Scopus)

Abstract

Tissue transglutaminase forms cross-links between lysine and glutamine side-chains of polypeptide chains in a Ca2+-dependent reaction; its structural basis is still not clarified. In this study, we demonstrate that the refolding of the human recombinant enzyme molecule to its catalytically active form from inclusion bodies needs the presence of a helper material with higher molecular mass, but only in the initiation phase. Ca2+ and nucleotides are ascribed as affector molecules also in the early phase of structural reconstitution. Two optimal concentrations of polyethylene glycol and a relatively long time scale for the evolution of the final structure were identified. The optimized refolding procedure is reported.

Original languageEnglish
Pages (from-to)59-70
Number of pages12
JournalPreparative Biochemistry and Biotechnology
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry

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