Polyamine titre in relation to chill-sensitivity in Phaseolus sp

M. G. Guye, L. Vigh, J. M. Wilson

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Abstract

Guye, M G., Vigh, L. and Wilson, J. M. 1986. Polyamine titre in relation to chill-sensitivity in Phaseolus sp.-J. exp. Bot. 37: 1036-1043.Endogenous levels of the polyamines putrescine, spermidine and spermine were quantified in the primary leaves of five cultivars of bean (Phaseolus sp.) differing in their 'wilting response' to a chilling exposure of 5 °C for 24 h.Levels of polyamines prior to chilling treatment did not appear to be correlated with chill-tolerance as levels in the non-chilled controls were highest in cultivars of medium chill-sensitivity. Plants grown under a vapour saturation deficit (VSD) of 8·4 gm-3 day/6·1 g m-3 night exhibited a mild hardening as compared to plants grown under a VSD of 5·7 gm-3 day/4·1 gm-3 night, as the former showed less wilting on chilling. Hardening at high VSD had the effect of slightly lowering the putrescine content of non-chilled tissue but total polyamine content remained unchanged. However, on chilling, the largest relative increase in polyamine levels, in particular that of putrescine, occurred in hardened plants. There was also a significant relative increase in putrescine titre in response to chilling in non-hardened genotypes of high chill-tolerance, whereas more chill-sensitive genotypes remained unchanged or slightly declined in putrescine content on chilling.Relative changes in putrescine content rather than absolute levels appears to be correlated with chill-tolerance. These results are discussed in view of present knowledge on the adaptive significance of stress-induced changes in polyamines, especially with regard to membrane stability.

Original languageEnglish
Pages (from-to)1036-1043
Number of pages8
JournalJournal of experimental botany
Volume37
Issue number7
DOIs
Publication statusPublished - Jul 1 1986

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Keywords

  • Chilling
  • Phaseolus sp.
  • Polyamines

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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