Polerovirus protein P0 prevents the assembly of small RNA-containing RISC complexes and leads to degradation of ARGONAUTE1

Tibor Csorba, Rita Lózsa, György Hutvágner, J. Burgyán

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

RNA silencing plays an important role in plants in defence against viruses. To overcome this defence, plant viruses encode suppressors of RNA silencing. The most common mode of silencing suppression is sequestration of double-stranded RNAs involved in the antiviral silencing pathways. Viral suppressors can also overcome silencing responses through protein-protein interaction. The poleroviral P0 silencing suppressor protein targets ARGONAUTE (AGO) proteins for degradation. AGO proteins are the core component of the RNA-induced silencing complex (RISC). We found that P0 does not interfere with the slicer activity of pre-programmed siRNA/miRNA containing AGO1, but prevents de novo formation of siRNA/miRNA containing AGO1. We show that the AGO1 protein is part of a high-molecular-weight complex, suggesting the existence of a multi-protein RISC in plants. We propose that P0 prevents RISC assembly by interacting with one of its protein components, thus inhibiting formation of siRNA/miRNA-RISC, and ultimately leading to AGO1 degradation. Our findings also suggest that siRNAs enhance the stability of co-expressed AGO1 in both the presence and absence of P0.

Original languageEnglish
Pages (from-to)463-472
Number of pages10
JournalPlant Journal
Volume62
Issue number3
DOIs
Publication statusPublished - May 2010

Fingerprint

Luteoviridae
Polerovirus
RNA-Induced Silencing Complex
Myelin P0 Protein
RNA
small interfering RNA
microRNA
degradation
MicroRNAs
Proteins
RNA interference
Small Interfering RNA
proteins
RNA Interference
plant viruses
double-stranded RNA
protein-protein interactions
protein degradation
Plant Viruses
Double-Stranded RNA

Keywords

  • AGO1
  • P0 silencing suppressor
  • Polerovirus
  • Protein ubiquitination
  • RISC assembly
  • RNA silencing

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Polerovirus protein P0 prevents the assembly of small RNA-containing RISC complexes and leads to degradation of ARGONAUTE1. / Csorba, Tibor; Lózsa, Rita; Hutvágner, György; Burgyán, J.

In: Plant Journal, Vol. 62, No. 3, 05.2010, p. 463-472.

Research output: Contribution to journalArticle

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