The polarization spectrum of the fluorescence emission at -140°C has been studied in the spectral range 670-760 nm using magnetically oriented spinach chloroplasts. With edge viewing oriented membranes the measurement of the fluorescence polarization (FP), a value reflecting the orientation of the emitting oscillators with respect to the membrane plane, indicates the existence of at least five different fluorescing species. The highest FP is found in the long wavelength band, the smallest in the 675 nm region. A dip at 695 nm is observed in the FP spectrum. A determination of the depolarization of the fluorescence by energy transfer measured with face viewing oriented membranes shows a very high memory of the polarization of the excitation in the long wavelength band of the emission spectrum.
|Number of pages||8|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - Aug 23 1976|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology