Polarized light spectroscopy on oriented spinach chloroplasts fluorescence emission at low temperature

Gy I. Garab, J. Breton

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The polarization spectrum of the fluorescence emission at -140°C has been studied in the spectral range 670-760 nm using magnetically oriented spinach chloroplasts. With edge viewing oriented membranes the measurement of the fluorescence polarization (FP), a value reflecting the orientation of the emitting oscillators with respect to the membrane plane, indicates the existence of at least five different fluorescing species. The highest FP is found in the long wavelength band, the smallest in the 675 nm region. A dip at 695 nm is observed in the FP spectrum. A determination of the depolarization of the fluorescence by energy transfer measured with face viewing oriented membranes shows a very high memory of the polarization of the excitation in the long wavelength band of the emission spectrum.

Original languageEnglish
Pages (from-to)1095-1102
Number of pages8
JournalBiochemical and biophysical research communications
Volume71
Issue number4
DOIs
Publication statusPublished - Aug 23 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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