Polarized and compartment-dependent distribution of HCN1 in pyramidal cell dendrites

Andrea Lörincz, Takuya Notomi, Gábor Tamás, Ryuichi Shigemoto, Zoltan Nusser

Research output: Contribution to journalArticle

303 Citations (Scopus)

Abstract

An ion channel's function depends largely on its location and density on neurons. Here we used high-resolution immunolocalization to determine the subcellular distribution of the hyperpolarization-activated and cyclic-nucleotide-gated channel subunit 1 (HCN1) in rat brain. Light microscopy revealed graded HCN1 immunoreactivity in apical dendrites of hippocampal, subicular and neocortical layer-5 pyramidal cells. Quantitative comparison of immunogold densities showed a 60-fold increase from somatic to distal apical dendritic membranes. Distal dendritic shafts had 16 times more HCN1 labeling than proximal dendrites of similar diameters. At the same distance from the soma, the density of HCN1 was significantly higher in dendritic shafts than in spines. Our results reveal the complex cell surface distribution of voltage-gated ion-channels, and predict its role in increasing the computational power of single neurons via subcellular domain and input-specific mechanisms.

Original languageEnglish
Pages (from-to)1185-1193
Number of pages9
JournalNature Neuroscience
Volume5
Issue number11
DOIs
Publication statusPublished - Nov 2002

ASJC Scopus subject areas

  • Neuroscience(all)

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