Polarity, spatial organisation of cytoskeleton, and nuclear division in morphologically altered cells of Schizosaccharomyces pombe

M. Sipiczki, A. Grallert

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To gain more information about the determination of cell polarity and its relationship to the organisation of cytoskeleton, we have examined the mycelial mutant sep1-1 and the multinucleate multipolar syncytia of the triple mutant se1-1 sepl1-1 cdc4-8 by indirect immunofluorescence techniques. We have found that polarity is predetermined by the shape of the fell. During transition from mitosis to interphase the microtubules of the arising cytoplasmic cytoskeleton gradually form a basket-like pattern that reflects the curvatures of the cell envelope. The presumable growing poles, where actin accumulates, usually correlates with the sites where the cell tapers and the microtubules converge. However, no growth can be launched at these sites if the cell surface has not been properly processed. Mitosis and meiosis are not affected significantly by changes in cell morphology and polarity, but larger cells are less effective during sporulation. The azygotic asci produced by multinucleate syncytia frequently contain over 20 ascospores.

Original languageEnglish
Pages (from-to)991-998
Number of pages8
JournalCanadian Journal of Microbiology
Volume43
Issue number11
Publication statusPublished - 1997

Fingerprint

Cell Nucleus Division
Schizosaccharomyces
Cytoskeleton
Actins
Poles
Cell Polarity
Giant Cells
Mitosis
Microtubules
Interphase
Meiosis
Indirect Fluorescent Antibody Technique
Growth

Keywords

  • Cell division cycle
  • Cytokinesis
  • Cytoskeleton
  • Fission yeast

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

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