Ploidy results in prostatic carcinoma vary with sampling method and with cytometric technique.

N. S. Hardt, J. B. Hendricks, Z. Sápi, G. Tykochinsky, E. J. Wilkinson, H. B. Epstein, Z. Wajsman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To evaluate the effect of sampling method and cytometric method on DNA ploidy results, a comparison study was performed on 20 whole prostate glands removed at prostatectomy. Fresh sampling was by sampling fine-needle aspiration (FNA). Paraffin sampling was by microdissection and re-embedding of 3 to 13 (average 6) 5-mm foci of microscopically proven tumor. Analysis was by flow cytometry and by image cytometry (microscopically guided). In tumor negative cases, flow and image cytometry of the FNA was diploid in each case, and flow cytometry of paraffin-embedded tissue was diploid in 5/6 cases. In tumor positive cases, non-diploid tumor was detected by image cytometry of the FNA in 70%, by flow cytometry of the FNA in 29%, and by flow cytometry of paraffin-extracted nuclei in 21%. The most effective combination was sampling fine-needle aspiration and image analysis.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalModern Pathology
Volume7
Issue number1
Publication statusPublished - Jan 1994

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Ploidies
Fine Needle Biopsy
Image Cytometry
Flow Cytometry
Carcinoma
Paraffin
Diploidy
Neoplasms
Microdissection
Prostatectomy
Prostate
DNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Hardt, N. S., Hendricks, J. B., Sápi, Z., Tykochinsky, G., Wilkinson, E. J., Epstein, H. B., & Wajsman, Z. (1994). Ploidy results in prostatic carcinoma vary with sampling method and with cytometric technique. Modern Pathology, 7(1), 44-48.

Ploidy results in prostatic carcinoma vary with sampling method and with cytometric technique. / Hardt, N. S.; Hendricks, J. B.; Sápi, Z.; Tykochinsky, G.; Wilkinson, E. J.; Epstein, H. B.; Wajsman, Z.

In: Modern Pathology, Vol. 7, No. 1, 01.1994, p. 44-48.

Research output: Contribution to journalArticle

Hardt, NS, Hendricks, JB, Sápi, Z, Tykochinsky, G, Wilkinson, EJ, Epstein, HB & Wajsman, Z 1994, 'Ploidy results in prostatic carcinoma vary with sampling method and with cytometric technique.', Modern Pathology, vol. 7, no. 1, pp. 44-48.
Hardt NS, Hendricks JB, Sápi Z, Tykochinsky G, Wilkinson EJ, Epstein HB et al. Ploidy results in prostatic carcinoma vary with sampling method and with cytometric technique. Modern Pathology. 1994 Jan;7(1):44-48.
Hardt, N. S. ; Hendricks, J. B. ; Sápi, Z. ; Tykochinsky, G. ; Wilkinson, E. J. ; Epstein, H. B. ; Wajsman, Z. / Ploidy results in prostatic carcinoma vary with sampling method and with cytometric technique. In: Modern Pathology. 1994 ; Vol. 7, No. 1. pp. 44-48.
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