Plethysmographic estimation of thoracic gas volume in apneic mice

Tibor Z. Jánosi, A. Adamicza, Graeme R. Zosky, Tibor Asztalos, Peter D. Sly, Z. Hantos

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Electrical stimulation of intercostal muscles was employed to measure thoracic gas volume (TGV) during airway occlusion in the absence of respiratory effort at different levels of lung inflation. In 15 tracheostomized and mechanically ventilated CBA/Ca mice, the value of TGV obtained from the spontaneous breathing effort available in the early phase of the experiments (TGVsp) was compared with those resulting from muscle stimulation (TGVst) at transrespiratory pressures of 0, 10, and 20 cmH2O. A very strong correlation (r2 = 0.97) was found, although with a systematically (∼16%) higher estimation of TGVst relative to TGVsp, attributable to the different durations of the stimulated (∼50 ms) and spontaneous (∼200 ms) contractions. Measurements of TGVst before and after injections of 0.2, 0.4, and 0.6 ml of nitrogen into the lungs in six mice resulted in good agreement between the change in TGVst and the injected volume (r2 = 0.98). In four mice, TGVsp and TGVst were compared at end expiration with air or a helium-oxygen mixture to confirm the validity of isothermal compression in the alveolar gas. The TGVst values measured at zero transrespiratory pressure in all CBA/Ca mice [0.29 ± 0.05 (SD) ml] and in C57BL/6 (N = 6; 0.34 = 0.08 ml) and BALB/c (N = 6; 0.28 ± 0.06 ml) mice were in agreement with functional residual capacity values from previous studies in which different techniques were used. This method is particularly useful when TGV is to be determined in the absence of breathing activity, when it must be known at any level of lung inflation or under non-steady-state conditions, such as during pharmaceutical interventions.

Original languageEnglish
Pages (from-to)454-459
Number of pages6
JournalJournal of Applied Physiology
Volume101
Issue number2
DOIs
Publication statusPublished - 2006

Fingerprint

Thorax
Gases
Inbred CBA Mouse
Economic Inflation
Lung
Respiration
Intercostal Muscles
Functional Residual Capacity
Pressure
Helium
Electric Stimulation
Nitrogen
Air
Oxygen
Muscles
Injections
Pharmaceutical Preparations

Keywords

  • Boyle's law
  • Functional residual capacity
  • Lung volume

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Plethysmographic estimation of thoracic gas volume in apneic mice. / Jánosi, Tibor Z.; Adamicza, A.; Zosky, Graeme R.; Asztalos, Tibor; Sly, Peter D.; Hantos, Z.

In: Journal of Applied Physiology, Vol. 101, No. 2, 2006, p. 454-459.

Research output: Contribution to journalArticle

Jánosi, Tibor Z. ; Adamicza, A. ; Zosky, Graeme R. ; Asztalos, Tibor ; Sly, Peter D. ; Hantos, Z. / Plethysmographic estimation of thoracic gas volume in apneic mice. In: Journal of Applied Physiology. 2006 ; Vol. 101, No. 2. pp. 454-459.
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