Platelet vinculin: a substrate of activated Factor XIII

Guus M. Asijee, L. Muszbek, J. Kappelmayer, János Polgár, Andrea Horváth, Auguste Sturk

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In addition to plasma, Factor XIII of blood coagulation (FXIII) is also present in the cytosol of platelets, monocytes and macrophages. However, its intracellular function has not yet been revealed. Activated Factor XIII (FXIIIa) is a transglutaminase (protein-glutamine: amine γ-glutamyltransferase, EC 2.3.2.13) of highly restricted substrate specificity with only a few known protein substrates. In this report, we showed that FXIIIa can link dansylcadaverine, radiolabelled histamine and putrescine to vinculin. Quantitative determinations revealed that in the vinculin molecule a single glutamine residue can serve as acyl donor for the incorporation of small-molecular-weight amines. Vinculin could not be crosslinked to another vinculin molecule. It could be covalently bound, however, to fibrinogen, which indicates that the acyl donor glutamine residue can be engaged in an ε-(γ-glutamyl)lysyl crosslink formation. Since it has been shown that platelet actin and myosin, two main components of cytoskeleton, are also substrates for FXIIIa, and that vinculin is associated to the cytoskeleton during platelet activation, the involvement of FXIII in the stabilization of cytoskeleton at certain phases of cellular function is a likely possibility.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume954
Issue numberC
DOIs
Publication statusPublished - 1988

Fingerprint

Factor XIIIa
Vinculin
Platelets
Blood Platelets
Glutamine
Cytoskeleton
Substrates
Amines
Factor XIII
Molecules
Transglutaminases
Putrescine
Macrophages
Platelet Activation
Myosins
Substrate Specificity
Cytosol
Fibrinogen
Histamine
Actins

Keywords

  • (Human)
  • Cytoskeleton
  • Factor XIII
  • Protein-glutamin γ-glutamyltransferase
  • Transglutaminase
  • Vinculin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

Cite this

Platelet vinculin : a substrate of activated Factor XIII. / Asijee, Guus M.; Muszbek, L.; Kappelmayer, J.; Polgár, János; Horváth, Andrea; Sturk, Auguste.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 954, No. C, 1988, p. 303-308.

Research output: Contribution to journalArticle

Asijee, Guus M. ; Muszbek, L. ; Kappelmayer, J. ; Polgár, János ; Horváth, Andrea ; Sturk, Auguste. / Platelet vinculin : a substrate of activated Factor XIII. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1988 ; Vol. 954, No. C. pp. 303-308.
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