Abstract
Fluorescence spectroscopy, a very sensitive index for measuring the biophysical properties of living cell systems, was used to examine the structural order of intact, resting, gel-filtered platelets from hyperlipidemic subjects (n = 48, 25-70 years) and normolipemic subjects (n = 34, 19-68 years). Fluorescence anisotropy (r[s]), which is inversely related to membrane fluidity, was estimated using 3 different fluorescent dyes, DPH, TMA-DPH, and 6-AS, known to label different regions of biological membranes. Increased membrane fluidity was observed in type IIB (n = 24, 36-62 yrs; r[s] = 0.0692 ± 0.09) and type IV (n = 10, 33-57 yrs; r[s] = 0.058 ± 0.006) hyperlipidemics in comparison to type IIA (n = 14, 25-70 yrs; r[s] = 0.086 ± 0.019) and control subjects (n = 24, 28-68 yrs; r[s] = 0.079 ± 0.012). The temperature dependency of r[s]-DPH values was significantly different (P <0.01) in platelets from type IIB and type IV patients compared to type IIA and control subjects of similar age. A significant positive correlation (P <0.005) between membrane fluidity and age was found only in healthy control subjects (n = 34, 19-68 yrs). Despite significant (P <0.01) differences in plasma lipid concentrations in hyperlipidemic patients and controls, significant ex vivo relations between membrane fluidity and lipoprotein concentrations, free fatty acid distribution, and increased age were found only in healthy control subjects. Plasma levels of thromboxane as well as serum selenium concentrations did not significantly differ between hypercholesterolemic, hypertriglyceridemic, and control subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 159-167 |
| Number of pages | 9 |
| Journal | Atherosclerosis |
| Volume | 87 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 1991 |
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Keywords
- Hyperlipoproteinemia
- Membrane fluidity
- Platelets
- Type IIA
- Type IIB
- Type IV
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Platelet membrane fluidity in type IIA, type IIB and type IV hyperlipoproteinemia. / Malle, Ernst; Sattler, Wolfgang; Prenner, Elmar; Leis, Hans J.; Karádi, I.; Knipping, Gabriele; Romics, Làszló; Kostner, Gerhard M.
In: Atherosclerosis, Vol. 87, No. 2-3, 1991, p. 159-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Platelet membrane fluidity in type IIA, type IIB and type IV hyperlipoproteinemia
AU - Malle, Ernst
AU - Sattler, Wolfgang
AU - Prenner, Elmar
AU - Leis, Hans J.
AU - Karádi, I.
AU - Knipping, Gabriele
AU - Romics, Làszló
AU - Kostner, Gerhard M.
PY - 1991
Y1 - 1991
N2 - Fluorescence spectroscopy, a very sensitive index for measuring the biophysical properties of living cell systems, was used to examine the structural order of intact, resting, gel-filtered platelets from hyperlipidemic subjects (n = 48, 25-70 years) and normolipemic subjects (n = 34, 19-68 years). Fluorescence anisotropy (r[s]), which is inversely related to membrane fluidity, was estimated using 3 different fluorescent dyes, DPH, TMA-DPH, and 6-AS, known to label different regions of biological membranes. Increased membrane fluidity was observed in type IIB (n = 24, 36-62 yrs; r[s] = 0.0692 ± 0.09) and type IV (n = 10, 33-57 yrs; r[s] = 0.058 ± 0.006) hyperlipidemics in comparison to type IIA (n = 14, 25-70 yrs; r[s] = 0.086 ± 0.019) and control subjects (n = 24, 28-68 yrs; r[s] = 0.079 ± 0.012). The temperature dependency of r[s]-DPH values was significantly different (P <0.01) in platelets from type IIB and type IV patients compared to type IIA and control subjects of similar age. A significant positive correlation (P <0.005) between membrane fluidity and age was found only in healthy control subjects (n = 34, 19-68 yrs). Despite significant (P <0.01) differences in plasma lipid concentrations in hyperlipidemic patients and controls, significant ex vivo relations between membrane fluidity and lipoprotein concentrations, free fatty acid distribution, and increased age were found only in healthy control subjects. Plasma levels of thromboxane as well as serum selenium concentrations did not significantly differ between hypercholesterolemic, hypertriglyceridemic, and control subjects.
AB - Fluorescence spectroscopy, a very sensitive index for measuring the biophysical properties of living cell systems, was used to examine the structural order of intact, resting, gel-filtered platelets from hyperlipidemic subjects (n = 48, 25-70 years) and normolipemic subjects (n = 34, 19-68 years). Fluorescence anisotropy (r[s]), which is inversely related to membrane fluidity, was estimated using 3 different fluorescent dyes, DPH, TMA-DPH, and 6-AS, known to label different regions of biological membranes. Increased membrane fluidity was observed in type IIB (n = 24, 36-62 yrs; r[s] = 0.0692 ± 0.09) and type IV (n = 10, 33-57 yrs; r[s] = 0.058 ± 0.006) hyperlipidemics in comparison to type IIA (n = 14, 25-70 yrs; r[s] = 0.086 ± 0.019) and control subjects (n = 24, 28-68 yrs; r[s] = 0.079 ± 0.012). The temperature dependency of r[s]-DPH values was significantly different (P <0.01) in platelets from type IIB and type IV patients compared to type IIA and control subjects of similar age. A significant positive correlation (P <0.005) between membrane fluidity and age was found only in healthy control subjects (n = 34, 19-68 yrs). Despite significant (P <0.01) differences in plasma lipid concentrations in hyperlipidemic patients and controls, significant ex vivo relations between membrane fluidity and lipoprotein concentrations, free fatty acid distribution, and increased age were found only in healthy control subjects. Plasma levels of thromboxane as well as serum selenium concentrations did not significantly differ between hypercholesterolemic, hypertriglyceridemic, and control subjects.
KW - Hyperlipoproteinemia
KW - Membrane fluidity
KW - Platelets
KW - Type IIA
KW - Type IIB
KW - Type IV
UR - http://www.scopus.com/inward/record.url?scp=0025811690&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025811690&partnerID=8YFLogxK
U2 - 10.1016/0021-9150(91)90018-X
DO - 10.1016/0021-9150(91)90018-X
M3 - Article
VL - 87
SP - 159
EP - 167
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 2-3
ER -