Platelet membrane fluidity in type IIA, type IIB and type IV hyperlipoproteinemia

Ernst Malle, Wolfgang Sattler, Elmar Prenner, Hans J. Leis, Istvàn Karàdi, Gabriele Knipping, Làszló Romics, Gerhard M. Kostner

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12 Citations (Scopus)

Abstract

Fluorescence spectroscopy, a very sensitive index for measuring the biophysical properties of living cell systems, was used to examine the structural order of intact, resting, gel-filtered platelets from hyperlipidemic subjects (n = 48, 25-70 years) and normolipemic subjects (n = 34, 19-68 years). Fluorescence anisotropy (r[s]), which is inversely related to membrane fluidity, was estimated using 3 different fluorescent dyes, DPH, TMA-DPH, and 6-AS, known to label different regions of biological membranes. Increased membrane fluidity was observed in type IIB (n = 24, 36-62 yrs; r[s] = 0.0692 ± 0.09) and type IV (n = 10, 33-57 yrs; r[s] = 0.058 ± 0.006) hyperlipidemics in comparison to type IIA (n = 14, 25-70 yrs; r[s] = 0.086 ± 0.019) and control subjects (n = 24, 28-68 yrs; r[s] = 0.079 ± 0.012). The temperature dependency of r[s]-DPH values was significantly different (P < 0.01) in platelets from type IIB and type IV patients compared to type IIA and control subjects of similar age. A significant positive correlation (P < 0.005) between membrane fluidity and age was found only in healthy control subjects (n = 34, 19-68 yrs). Despite significant (P < 0.01) differences in plasma lipid concentrations in hyperlipidemic patients and controls, significant ex vivo relations between membrane fluidity and lipoprotein concentrations, free fatty acid distribution, and increased age were found only in healthy control subjects. Plasma levels of thromboxane as well as serum selenium concentrations did not significantly differ between hypercholesterolemic, hypertriglyceridemic, and control subjects.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalAtherosclerosis
Volume87
Issue number2-3
DOIs
Publication statusPublished - Apr 1991

Keywords

  • Hyperlipoproteinemia
  • Membrane fluidity
  • Platelets
  • Type IIA
  • Type IIB
  • Type IV

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Malle, E., Sattler, W., Prenner, E., Leis, H. J., Karàdi, I., Knipping, G., Romics, L., & Kostner, G. M. (1991). Platelet membrane fluidity in type IIA, type IIB and type IV hyperlipoproteinemia. Atherosclerosis, 87(2-3), 159-167. https://doi.org/10.1016/0021-9150(91)90018-X