Platelet-derived microparticle levels are significantly elevated in patients treated by elective stenting compared to subjects with diagnostic catheterization alone

Béla Nagy, Tibor Szúk, Ildikó Beke Debreceni, János Kappelmayer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Significant platelet reactivity with endothelial damage may occur during percutaneous coronary intervention such as balloon angioplasty and elective stenting in patients with stenotic or occluded arteries in coronary artery disease. Activated platelets express several surface epitopes to aggregate and form heterotypic interactions with leukocytes and endothelial cells, secrete biomarkers to enhance their prothrombotic properties and also generate increased level of microparticles (PMPs). These events may contribute to subacute stent thrombosis induced by the procedure-mediated trauma. Our aim was to study the level of PMPs and other platelet activation markers at an early time point after stenting with bare metal stent in patients on aspirin antiplatelet pre-treatment, and these results were compared to data obtained from subjects with diagnostic catheterization alone. We found that at 15 minutes after the completion of stenting, the levels of PMPs (557 ± 83/μl versus 325 ± 42/μl), the platelet P-selectin expression (2.7 ± 0.3 versus 1.99 ± 0.1) and the ratio of platelet-monocyte heterotypic aggregates (48 ± 4 versus 38 ± 3) were significantly (p <0.05) elevated in patients compared to unstented subjects, but no difference was found in soluble P-selectin values. We suggest that the observed cellular changes are early and sensitive markers to detect the platelet-activating effect of stent implantation.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalPlatelets
Volume21
Issue number2
DOIs
Publication statusPublished - 2010

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Catheterization
Blood Platelets
Stents
P-Selectin
Balloon Angioplasty
Platelet Activation
Percutaneous Coronary Intervention
Aspirin
Coronary Artery Disease
Epitopes
Monocytes
Thrombosis
Leukocytes
Endothelial Cells
Arteries
Biomarkers
Metals
Wounds and Injuries
Therapeutics

Keywords

  • Flow cytometry
  • Microparticles
  • Platelet activation
  • Stent

ASJC Scopus subject areas

  • Hematology

Cite this

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abstract = "Significant platelet reactivity with endothelial damage may occur during percutaneous coronary intervention such as balloon angioplasty and elective stenting in patients with stenotic or occluded arteries in coronary artery disease. Activated platelets express several surface epitopes to aggregate and form heterotypic interactions with leukocytes and endothelial cells, secrete biomarkers to enhance their prothrombotic properties and also generate increased level of microparticles (PMPs). These events may contribute to subacute stent thrombosis induced by the procedure-mediated trauma. Our aim was to study the level of PMPs and other platelet activation markers at an early time point after stenting with bare metal stent in patients on aspirin antiplatelet pre-treatment, and these results were compared to data obtained from subjects with diagnostic catheterization alone. We found that at 15 minutes after the completion of stenting, the levels of PMPs (557 ± 83/μl versus 325 ± 42/μl), the platelet P-selectin expression (2.7 ± 0.3 versus 1.99 ± 0.1) and the ratio of platelet-monocyte heterotypic aggregates (48 ± 4 versus 38 ± 3) were significantly (p <0.05) elevated in patients compared to unstented subjects, but no difference was found in soluble P-selectin values. We suggest that the observed cellular changes are early and sensitive markers to detect the platelet-activating effect of stent implantation.",
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AU - Nagy, Béla

AU - Szúk, Tibor

AU - Debreceni, Ildikó Beke

AU - Kappelmayer, János

PY - 2010

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