Plasminogen activator activity and plasminogen independent amidolytic activity in tear fluid from healthy persons and patients with anterior segment inflammation

J. Tőzsér, A. Berta, Mária Punyiczki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Plasminogen activator activity and plasminogen independent amidolytic activity were measured in human tears by a spectrophotometric method using human plasminogen and chromogenic peptide substrate S-2251. This assay is sensitive predominantly to urokinase-like plasminogen activator. Tears were collected with glass capillaries. The activator activity in normal tears was found to be low, 0.06 ± 0.04 (SD) IU/ml. Elevated levels were measured in the tears of patients with various types of conjunctival and corneal disorders. The affected epithelial cells of the cornea and conjunctiva were suggested to be responsible for the elevated activity. Plasminogen independent amidolytic activity was usually very low except in cases of increased permeability of the conjunctival blood vessels. The procedure is recommended as a useful tool for the study of the pathological changes in the epithelial cells of the cornea and conjunctiva.

Original languageEnglish
Pages (from-to)323-331
Number of pages9
JournalClinica Chimica Acta
Volume183
Issue number3
DOIs
Publication statusPublished - Aug 31 1989

Fingerprint

Plasminogen Activators
Plasminogen
Tears
Inflammation
Fluids
Conjunctiva
Chromogenics
Cornea
valyl-leucyl-lysine 4-nitroanilide
Epithelial Cells
Urokinase-Type Plasminogen Activator
Blood vessels
Chromogenic Compounds
Assays
Glass
Blood Vessels
Permeability
Peptides
Substrates

Keywords

  • Chromogenic method
  • Corneal and conjunctival diseases
  • Plasmin
  • Plasminogen activators
  • Tears

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

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abstract = "Plasminogen activator activity and plasminogen independent amidolytic activity were measured in human tears by a spectrophotometric method using human plasminogen and chromogenic peptide substrate S-2251. This assay is sensitive predominantly to urokinase-like plasminogen activator. Tears were collected with glass capillaries. The activator activity in normal tears was found to be low, 0.06 ± 0.04 (SD) IU/ml. Elevated levels were measured in the tears of patients with various types of conjunctival and corneal disorders. The affected epithelial cells of the cornea and conjunctiva were suggested to be responsible for the elevated activity. Plasminogen independent amidolytic activity was usually very low except in cases of increased permeability of the conjunctival blood vessels. The procedure is recommended as a useful tool for the study of the pathological changes in the epithelial cells of the cornea and conjunctiva.",
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AU - Tőzsér, J.

AU - Berta, A.

AU - Punyiczki, Mária

PY - 1989/8/31

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N2 - Plasminogen activator activity and plasminogen independent amidolytic activity were measured in human tears by a spectrophotometric method using human plasminogen and chromogenic peptide substrate S-2251. This assay is sensitive predominantly to urokinase-like plasminogen activator. Tears were collected with glass capillaries. The activator activity in normal tears was found to be low, 0.06 ± 0.04 (SD) IU/ml. Elevated levels were measured in the tears of patients with various types of conjunctival and corneal disorders. The affected epithelial cells of the cornea and conjunctiva were suggested to be responsible for the elevated activity. Plasminogen independent amidolytic activity was usually very low except in cases of increased permeability of the conjunctival blood vessels. The procedure is recommended as a useful tool for the study of the pathological changes in the epithelial cells of the cornea and conjunctiva.

AB - Plasminogen activator activity and plasminogen independent amidolytic activity were measured in human tears by a spectrophotometric method using human plasminogen and chromogenic peptide substrate S-2251. This assay is sensitive predominantly to urokinase-like plasminogen activator. Tears were collected with glass capillaries. The activator activity in normal tears was found to be low, 0.06 ± 0.04 (SD) IU/ml. Elevated levels were measured in the tears of patients with various types of conjunctival and corneal disorders. The affected epithelial cells of the cornea and conjunctiva were suggested to be responsible for the elevated activity. Plasminogen independent amidolytic activity was usually very low except in cases of increased permeability of the conjunctival blood vessels. The procedure is recommended as a useful tool for the study of the pathological changes in the epithelial cells of the cornea and conjunctiva.

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