Plasminogen activator activity and inhibition in rabbit tears after photorefractive keratectomy

Adrienne Csutak, David M. Silver, J. Tőzsér, Andrea Facskó, A. Berta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Plasminogen activator is a normal component of tear fluid that plays a role in corneal wound healing processes. This work examines whether inhibitor-induced low levels of plasminogen activator activity (PAA) during corneal re-epithelialization after excimer laser photorefractive keratectomy (PRK) correlates with the eventual occurrence of haze in rabbit eyes. Tear samples were collected with glass capillaries from 16 eyes of eight New Zealand rabbits, using i.m. injection of pilocarpine hydrochloride for stimulation. Tears were collected before and after PRK surgery, and then daily for 5 days, and every fourth day thereafter for 3 months. Both eyes underwent PRK treatment. One eye of each rabbit was treated as a control while the contralateral eye was treated with aprotinin, a serine protease inhibitor, over the first 7 days. PAA in the tear samples was measured by a spectrophotometric method using human plasminogen and chromogenic peptide substrate S-2251. For the eight control eyes after PRK, the PAA values were significantly lower (day 1) and higher (days 2 and 3) than the equilibrium PAA (p

Original languageEnglish
Pages (from-to)675-680
Number of pages6
JournalExperimental Eye Research
Volume77
Issue number6
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Photorefractive Keratectomy
Plasminogen Activators
Tears
Rabbits
valyl-leucyl-lysine 4-nitroanilide
Laser Corneal Surgery
Re-Epithelialization
Chromogenic Compounds
Excimer Lasers
Serine Proteinase Inhibitors
Aprotinin
Pilocarpine
Plasminogen
Wound Healing
Glass
Peptides
Injections

Keywords

  • Corneal wound healing
  • Photorefractive keratectomy
  • Plasminogen activator
  • Rabbit tears
  • Refractive surgery

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Plasminogen activator activity and inhibition in rabbit tears after photorefractive keratectomy. / Csutak, Adrienne; Silver, David M.; Tőzsér, J.; Facskó, Andrea; Berta, A.

In: Experimental Eye Research, Vol. 77, No. 6, 12.2003, p. 675-680.

Research output: Contribution to journalArticle

Csutak, Adrienne ; Silver, David M. ; Tőzsér, J. ; Facskó, Andrea ; Berta, A. / Plasminogen activator activity and inhibition in rabbit tears after photorefractive keratectomy. In: Experimental Eye Research. 2003 ; Vol. 77, No. 6. pp. 675-680.
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