Plasmid-mediated quinolone resistance among extended-spectrum beta-lactamase producing enterobacteriaceae from bloodstream infections

Judit Domokos, Katalin Kristóf, D. Szabó

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The purpose of this study was to determine prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes [qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')-Ib-cr, qepA, and oqxAB] among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. isolates from bloodcultures in Hungary. A total of 103 isolates were tested for quinolone susceptibility by microdilution method and PMQR genes were detected by polymerase chain reaction. About 40 ESBL-producing E. coli (39%) and 50 ESBL-producing Klebsiella spp. strains (48%) were resistant to ciprofloxacin; 40 ESBL-producing E. coli (39%) and 47 ESBL-producing Klebsiella spp. strains (45%) were resistant to levofloxacin; and 88 strains including 40 ESBL-producing E. coli (39%) and 48 (47%) ESBL-producing Klebsiella spp. were resistant to moxifloxacin. Among the 103 ESBL-producing isolates, 77 (75%) isolates (30 E. coli and 47 Klebsiella spp.) harbored PMQR genes. The most commonly detected gene was aac(6')-Ib-cr (65%). The occurrence of qnrS gene was 6%. Interestingly, qnrA, qnrB, qnrC, qnrD, and qepA were not found in any isolates. Among 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried two and three different PMQR genes, respectively. Only Klebsiella spp. harbored more than one PMQR genes. Observing prevalence of PMQR genes in the last 8 years, the increasing incidence of aac(6')-Ib-cr and oqxAB can be seen. Our results highlight high frequency of PMQR genes among ESBL-producing Klebsiella pneumoniae and E. coli isolates with an increasing dynamics in Hungary.

Original languageEnglish
Pages (from-to)313-323
Number of pages11
JournalActa Microbiologica et Immunologica Hungarica
Volume63
Issue number3
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Enterobacteriaceae Infections
Quinolones
beta-Lactamases
Plasmids
Klebsiella
Escherichia coli
Genes
Hungary
Levofloxacin
Klebsiella pneumoniae
Ciprofloxacin

Keywords

  • Aac(6')-Ib-cr
  • OqxAB
  • Plasmid-mediated quinolone resistance
  • Qnr

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)

Cite this

Plasmid-mediated quinolone resistance among extended-spectrum beta-lactamase producing enterobacteriaceae from bloodstream infections. / Domokos, Judit; Kristóf, Katalin; Szabó, D.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 63, No. 3, 01.09.2016, p. 313-323.

Research output: Contribution to journalArticle

@article{5f648c4ae6af46ce8bdf5954b368f121,
title = "Plasmid-mediated quinolone resistance among extended-spectrum beta-lactamase producing enterobacteriaceae from bloodstream infections",
abstract = "The purpose of this study was to determine prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes [qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')-Ib-cr, qepA, and oqxAB] among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. isolates from bloodcultures in Hungary. A total of 103 isolates were tested for quinolone susceptibility by microdilution method and PMQR genes were detected by polymerase chain reaction. About 40 ESBL-producing E. coli (39{\%}) and 50 ESBL-producing Klebsiella spp. strains (48{\%}) were resistant to ciprofloxacin; 40 ESBL-producing E. coli (39{\%}) and 47 ESBL-producing Klebsiella spp. strains (45{\%}) were resistant to levofloxacin; and 88 strains including 40 ESBL-producing E. coli (39{\%}) and 48 (47{\%}) ESBL-producing Klebsiella spp. were resistant to moxifloxacin. Among the 103 ESBL-producing isolates, 77 (75{\%}) isolates (30 E. coli and 47 Klebsiella spp.) harbored PMQR genes. The most commonly detected gene was aac(6')-Ib-cr (65{\%}). The occurrence of qnrS gene was 6{\%}. Interestingly, qnrA, qnrB, qnrC, qnrD, and qepA were not found in any isolates. Among 77 PMQR-positive isolates, 27 (35.1{\%}) and 1 (1.3{\%}) carried two and three different PMQR genes, respectively. Only Klebsiella spp. harbored more than one PMQR genes. Observing prevalence of PMQR genes in the last 8 years, the increasing incidence of aac(6')-Ib-cr and oqxAB can be seen. Our results highlight high frequency of PMQR genes among ESBL-producing Klebsiella pneumoniae and E. coli isolates with an increasing dynamics in Hungary.",
keywords = "Aac(6')-Ib-cr, OqxAB, Plasmid-mediated quinolone resistance, Qnr",
author = "Judit Domokos and Katalin Krist{\'o}f and D. Szab{\'o}",
year = "2016",
month = "9",
day = "1",
doi = "10.1556/030.63.2016.002",
language = "English",
volume = "63",
pages = "313--323",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Plasmid-mediated quinolone resistance among extended-spectrum beta-lactamase producing enterobacteriaceae from bloodstream infections

AU - Domokos, Judit

AU - Kristóf, Katalin

AU - Szabó, D.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - The purpose of this study was to determine prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes [qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')-Ib-cr, qepA, and oqxAB] among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. isolates from bloodcultures in Hungary. A total of 103 isolates were tested for quinolone susceptibility by microdilution method and PMQR genes were detected by polymerase chain reaction. About 40 ESBL-producing E. coli (39%) and 50 ESBL-producing Klebsiella spp. strains (48%) were resistant to ciprofloxacin; 40 ESBL-producing E. coli (39%) and 47 ESBL-producing Klebsiella spp. strains (45%) were resistant to levofloxacin; and 88 strains including 40 ESBL-producing E. coli (39%) and 48 (47%) ESBL-producing Klebsiella spp. were resistant to moxifloxacin. Among the 103 ESBL-producing isolates, 77 (75%) isolates (30 E. coli and 47 Klebsiella spp.) harbored PMQR genes. The most commonly detected gene was aac(6')-Ib-cr (65%). The occurrence of qnrS gene was 6%. Interestingly, qnrA, qnrB, qnrC, qnrD, and qepA were not found in any isolates. Among 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried two and three different PMQR genes, respectively. Only Klebsiella spp. harbored more than one PMQR genes. Observing prevalence of PMQR genes in the last 8 years, the increasing incidence of aac(6')-Ib-cr and oqxAB can be seen. Our results highlight high frequency of PMQR genes among ESBL-producing Klebsiella pneumoniae and E. coli isolates with an increasing dynamics in Hungary.

AB - The purpose of this study was to determine prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes [qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')-Ib-cr, qepA, and oqxAB] among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. isolates from bloodcultures in Hungary. A total of 103 isolates were tested for quinolone susceptibility by microdilution method and PMQR genes were detected by polymerase chain reaction. About 40 ESBL-producing E. coli (39%) and 50 ESBL-producing Klebsiella spp. strains (48%) were resistant to ciprofloxacin; 40 ESBL-producing E. coli (39%) and 47 ESBL-producing Klebsiella spp. strains (45%) were resistant to levofloxacin; and 88 strains including 40 ESBL-producing E. coli (39%) and 48 (47%) ESBL-producing Klebsiella spp. were resistant to moxifloxacin. Among the 103 ESBL-producing isolates, 77 (75%) isolates (30 E. coli and 47 Klebsiella spp.) harbored PMQR genes. The most commonly detected gene was aac(6')-Ib-cr (65%). The occurrence of qnrS gene was 6%. Interestingly, qnrA, qnrB, qnrC, qnrD, and qepA were not found in any isolates. Among 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried two and three different PMQR genes, respectively. Only Klebsiella spp. harbored more than one PMQR genes. Observing prevalence of PMQR genes in the last 8 years, the increasing incidence of aac(6')-Ib-cr and oqxAB can be seen. Our results highlight high frequency of PMQR genes among ESBL-producing Klebsiella pneumoniae and E. coli isolates with an increasing dynamics in Hungary.

KW - Aac(6')-Ib-cr

KW - OqxAB

KW - Plasmid-mediated quinolone resistance

KW - Qnr

UR - http://www.scopus.com/inward/record.url?scp=84990180262&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990180262&partnerID=8YFLogxK

U2 - 10.1556/030.63.2016.002

DO - 10.1556/030.63.2016.002

M3 - Article

VL - 63

SP - 313

EP - 323

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 3

ER -