Plasma osteopontin concentrations in preeclampsia - Is there an association with endothelial injury?

Balázs Stenczer, J. Rigó, Z. Prohászka, Zoltán Derzsy, Levente Lázár, Veronika Makó, L. Cervenák, K. Balogh, M. Mézes, I. Karádi, A. Molvarec

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: It has been previously reported that plasma osteopontin (OPN) concentrations are increased in cardiovascular disorders. The goal of the present study was to determine plasma OPN concentrations in healthy pregnant women and preeclamptic patients, and to investigate their relationship to the clinical characteristics of the study subjects and to markers of inflammation [C-reactive protein (CRP)], endothelial activation [von Willebrand factor antigen (VWF:Ag)] or endothelial injury (fibronectin), oxidative stress [malondialdehyde (MDA)] and trophoblast debris (cell-free fetal DNA). Methods: Forty-four patients with preeclampsia and 44 healthy pregnant women matched for age and gestational age were involved in this case-control study. Plasma OPN concentrations were measured with ELISA. Serum CRP concentrations were determined with an autoanalyzer using the manufacturer's reagents. Plasma VWF:Ag was quantified by ELISA, while plasma fibronectin concentrations were measured by nephelometry. Plasma MDA concentrations were estimated by the thiobarbituric acid-based colorimetric assay. The amount of cell-free fetal DNA in maternal plasma was determined by quantitative real-time PCR analysis of the sex-determining region Y (SRY) gene. For statistical analyses, non-parametric methods were applied. Results: Serum levels of CRP, as well as plasma concentrations of VWF:Ag, fibronectin, MDA and cell-free fetal DNA were significantly higher in preeclamptic patients than in healthy pregnant women. There was no significant difference in plasma OPN concentrations between controls and the preeclamptic group. However, preeclamptic patients with plasma fibronectin concentrations in the upper quartile had significantly higher plasma OPN concentrations than those below the 75th percentile, as well as healthy pregnant women [median (interquartile range): 9.38 (8.10-11.99) vs. 7.54 (6.31-9.40) and 7.40 (6.51-8.80) ng/mL, respectively, p

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume48
Issue number2
DOIs
Publication statusPublished - Feb 1 2010

Fingerprint

Osteopontin
Pre-Eclampsia
Association reactions
Plasmas
Wounds and Injuries
Fibronectins
Pregnant Women
von Willebrand Factor
Malondialdehyde
C-Reactive Protein
Antigens
DNA
sry Genes
Enzyme-Linked Immunosorbent Assay
Nephelometry and Turbidimetry
Oxidative stress
Trophoblasts
Debris
Gestational Age
Case-Control Studies

Keywords

  • Endothelial injury
  • Fibronectin
  • Osteopontin
  • Preeclampsia
  • Pregnancy

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Plasma osteopontin concentrations in preeclampsia - Is there an association with endothelial injury? / Stenczer, Balázs; Rigó, J.; Prohászka, Z.; Derzsy, Zoltán; Lázár, Levente; Makó, Veronika; Cervenák, L.; Balogh, K.; Mézes, M.; Karádi, I.; Molvarec, A.

In: Clinical Chemistry and Laboratory Medicine, Vol. 48, No. 2, 01.02.2010, p. 181-187.

Research output: Contribution to journalArticle

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AU - Lázár, Levente

AU - Makó, Veronika

AU - Cervenák, L.

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