Plasma membrane Ca2+ pump isoforms 2a and 2b are unusually responsive to calmodulin and Ca2+

Nancy L. Elwess, Adelaida G. Filoteo, A. Enyedi, John T. Penniston

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The full-length a and b variants of the rat plasma membrane calcium pump, isoform 2 (rPMCA2a and rPMCA2b), were constructed and expressed in COS- 7 cells. To characterize these isoforms, calcium transport was determined in a microsomal fraction. Both rPMCA2a and rPMCA2b had a much higher affinity for calmodulin than the corresponding forms of hPMCA4, and rPMCA2b had the highest affinity among the isoforms that have been tested so far. When analyzed at a relatively high calmodulin concentration, rPMCA2b and, to a lesser extent, rPMCA2a showed higher apparent calcium affinity; i.e. they were more active at lower Ca2+ concentrations than hPMCA4b. This indicates that these two variants of rat isoform 2 will tend to maintain a lower free cytosolic Ca2+ level in cells where they are expressed. Both variants also showed a higher level of basal activity (in the complete absence of calmodulin) than hPMCA4b, a property which would reinforce their ability to maintain a low free cytosolic Ca2+ concentration. Experiments designed to determine the source of the higher apparent Ca2+ affinity of rPMCA2b showed that it came from the properties of the carboxyl terminus, rather than from any difference in the catalytic core.

Original languageEnglish
Pages (from-to)17981-17986
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number29
DOIs
Publication statusPublished - Jul 18 1997

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Calmodulin
Cell membranes
Protein Isoforms
Cell Membrane
Pumps
Calcium
Rats
COS Cells
Catalytic Domain
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Plasma membrane Ca2+ pump isoforms 2a and 2b are unusually responsive to calmodulin and Ca2+. / Elwess, Nancy L.; Filoteo, Adelaida G.; Enyedi, A.; Penniston, John T.

In: Journal of Biological Chemistry, Vol. 272, No. 29, 18.07.1997, p. 17981-17986.

Research output: Contribution to journalArticle

Elwess, Nancy L. ; Filoteo, Adelaida G. ; Enyedi, A. ; Penniston, John T. / Plasma membrane Ca2+ pump isoforms 2a and 2b are unusually responsive to calmodulin and Ca2+. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 29. pp. 17981-17986.
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