Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies

Adelaida G. Filoteo, Nancy L. Elwess, A. Enyedi, Ariel Caride, Htet Htet Aung, John T. Penniston

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The expression at the protein level of plasma membrane calcium pump (PMCA) isoforms in rat brain was detected by new antibodies that distinguished the four gene products and their alternatively spliced variants. All four gene products were distributed throughout hippocampus, cortex, and cerebellum, but the alternate splices showed more distinct distribution patterns. The b splice of isoform 1 was not detectable in any of the brain regions, which makes it unlikely that this isoform performs an essential housekeeping role as is frequently proposed. The b splices of isoforms 3 and 4, although expressed in all three regions, showed evidence of proteolysis, which removed a portion of the carboxyl terminus. In contrast, isoform 2b retained its full length, indicating that PMCA2b is more resistant to proteolysis than the other b forms. Whereas substantial amounts of isoforms 1a, 2a, and 3a were expressed in all regions, 4a was found only in frontal cortex. The distinct patterns of expression of the PMCA isoforms in brain suggest that some of them play a special role in intracellular Ca regulation.

Original languageEnglish
Pages (from-to)23741-23747
Number of pages7
JournalJournal of Biological Chemistry
Volume272
Issue number38
DOIs
Publication statusPublished - Sep 19 1997

Fingerprint

Cell membranes
Rats
Brain
Protein Isoforms
Cell Membrane
Pumps
Antibodies
Proteolysis
Genes
Calcium
Housekeeping
Frontal Lobe
Cerebellum
Hippocampus
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. / Filoteo, Adelaida G.; Elwess, Nancy L.; Enyedi, A.; Caride, Ariel; Aung, Htet Htet; Penniston, John T.

In: Journal of Biological Chemistry, Vol. 272, No. 38, 19.09.1997, p. 23741-23747.

Research output: Contribution to journalArticle

Filoteo, Adelaida G. ; Elwess, Nancy L. ; Enyedi, A. ; Caride, Ariel ; Aung, Htet Htet ; Penniston, John T. / Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 38. pp. 23741-23747.
@article{1ea99eda4f58496c8ea615cca4892414,
title = "Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies",
abstract = "The expression at the protein level of plasma membrane calcium pump (PMCA) isoforms in rat brain was detected by new antibodies that distinguished the four gene products and their alternatively spliced variants. All four gene products were distributed throughout hippocampus, cortex, and cerebellum, but the alternate splices showed more distinct distribution patterns. The b splice of isoform 1 was not detectable in any of the brain regions, which makes it unlikely that this isoform performs an essential housekeeping role as is frequently proposed. The b splices of isoforms 3 and 4, although expressed in all three regions, showed evidence of proteolysis, which removed a portion of the carboxyl terminus. In contrast, isoform 2b retained its full length, indicating that PMCA2b is more resistant to proteolysis than the other b forms. Whereas substantial amounts of isoforms 1a, 2a, and 3a were expressed in all regions, 4a was found only in frontal cortex. The distinct patterns of expression of the PMCA isoforms in brain suggest that some of them play a special role in intracellular Ca regulation.",
author = "Filoteo, {Adelaida G.} and Elwess, {Nancy L.} and A. Enyedi and Ariel Caride and Aung, {Htet Htet} and Penniston, {John T.}",
year = "1997",
month = "9",
day = "19",
doi = "10.1074/jbc.272.38.23741",
language = "English",
volume = "272",
pages = "23741--23747",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "38",

}

TY - JOUR

T1 - Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies

AU - Filoteo, Adelaida G.

AU - Elwess, Nancy L.

AU - Enyedi, A.

AU - Caride, Ariel

AU - Aung, Htet Htet

AU - Penniston, John T.

PY - 1997/9/19

Y1 - 1997/9/19

N2 - The expression at the protein level of plasma membrane calcium pump (PMCA) isoforms in rat brain was detected by new antibodies that distinguished the four gene products and their alternatively spliced variants. All four gene products were distributed throughout hippocampus, cortex, and cerebellum, but the alternate splices showed more distinct distribution patterns. The b splice of isoform 1 was not detectable in any of the brain regions, which makes it unlikely that this isoform performs an essential housekeeping role as is frequently proposed. The b splices of isoforms 3 and 4, although expressed in all three regions, showed evidence of proteolysis, which removed a portion of the carboxyl terminus. In contrast, isoform 2b retained its full length, indicating that PMCA2b is more resistant to proteolysis than the other b forms. Whereas substantial amounts of isoforms 1a, 2a, and 3a were expressed in all regions, 4a was found only in frontal cortex. The distinct patterns of expression of the PMCA isoforms in brain suggest that some of them play a special role in intracellular Ca regulation.

AB - The expression at the protein level of plasma membrane calcium pump (PMCA) isoforms in rat brain was detected by new antibodies that distinguished the four gene products and their alternatively spliced variants. All four gene products were distributed throughout hippocampus, cortex, and cerebellum, but the alternate splices showed more distinct distribution patterns. The b splice of isoform 1 was not detectable in any of the brain regions, which makes it unlikely that this isoform performs an essential housekeeping role as is frequently proposed. The b splices of isoforms 3 and 4, although expressed in all three regions, showed evidence of proteolysis, which removed a portion of the carboxyl terminus. In contrast, isoform 2b retained its full length, indicating that PMCA2b is more resistant to proteolysis than the other b forms. Whereas substantial amounts of isoforms 1a, 2a, and 3a were expressed in all regions, 4a was found only in frontal cortex. The distinct patterns of expression of the PMCA isoforms in brain suggest that some of them play a special role in intracellular Ca regulation.

UR - http://www.scopus.com/inward/record.url?scp=0030612653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030612653&partnerID=8YFLogxK

U2 - 10.1074/jbc.272.38.23741

DO - 10.1074/jbc.272.38.23741

M3 - Article

C2 - 9295318

AN - SCOPUS:0030612653

VL - 272

SP - 23741

EP - 23747

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 38

ER -