Plasma lactoferrin levels in leukaemias

K. Német, V. Solti, A. Mod, K. Pálóczi, G. Szegedi, Z. Hollán

Research output: Contribution to journalArticle

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Abstract

A solid-phase, one-step radioimmunoassay was developed for the determination of plasma lactoferrin concentration. The detection limit of the assay is 150 μg/l. Leakage of cellular lactoferrin was minimal when EDTA was used as anticoagulant, while results obtained from serum and from heparinized plasma were not reproducible. The plasma lactoferrin concentration of 35 female and 44 male healthy adults was measured in order to determine normal values. The geometric mean of lactoferrin levels in men is about 10% higher than in women: 483 (200-1500) μg/l in men and 446 (200-870) μg/l in women. Patients with acute and chronic leukaemias were also studied. In 38 patients with chronic myeloid leukaemia plasma lactoferrin levels were increased by three times while the neutrophil count was ten times higher than normal. Normal lactoferrin concentrations were measured in plasma samples from 15 patients with chronic lymphocytic leukaemia in incomplete remission while no detectable lactoferrin was found in samples from those in relapse (10 patients). In the untreated patients or those in relapse (19 cases) of both acute lymphocytic and myeloid leukaemias, plasma lactoferrin concentrations were undetectable while they seemed to return to normal during remission (3 cases). The data obtained indicate that the determination of plasma lactoferrin concentration might play an important role in facilitating the assessment of total blood granulocyte pool (TBGP).

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalClinical and Laboratory Haematology
Volume9
Issue number2
Publication statusPublished - 1987

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Lactoferrin
Leukemia
Plasmas
Recurrence
B-Cell Chronic Lymphocytic Leukemia
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Granulocytes
Acute Myeloid Leukemia
Edetic Acid
Anticoagulants
Radioimmunoassay
Limit of Detection
Assays
Reference Values
Neutrophils
Blood

ASJC Scopus subject areas

  • Hematology

Cite this

Plasma lactoferrin levels in leukaemias. / Német, K.; Solti, V.; Mod, A.; Pálóczi, K.; Szegedi, G.; Hollán, Z.

In: Clinical and Laboratory Haematology, Vol. 9, No. 2, 1987, p. 137-145.

Research output: Contribution to journalArticle

Német, K. ; Solti, V. ; Mod, A. ; Pálóczi, K. ; Szegedi, G. ; Hollán, Z. / Plasma lactoferrin levels in leukaemias. In: Clinical and Laboratory Haematology. 1987 ; Vol. 9, No. 2. pp. 137-145.
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