Plasma concentration of myeloperoxidase enzyme in pre- and post-climacterial people: Related superoxide anion generation

Gábor Békési, Réka Kakucs, József Sándor, E. Sárváry, I. Kocsis, Detlef Sprintz, S. Várbíró, Zoltán Magyar, A. Hrabák, J. Fehér, B. Székács

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Neutrophil granulocytes are involved in the pathogenesis of atherosclerosis also through their free radical generation. The aim of the study was to test how extracellular levels of myeloperoxidase (MPO; a granulocyte enzyme playing role in free radical production) change by age and what effect this change has on the production of the free radical superoxide anion by neutrophils. We also wanted to examine whether the antioxidant effect of different steroid hormones is realized through the MPO. Plasma myeloperoxidase concentrations of healthy blood donors were quantified by ELISA. Superoxide anion production was measured by photometry. Myeloperoxidase concentration was significantly lower in plasmas obtained from older women and men than in those from younger subjects. Adding the MPO inhibitors 4-aminobenzoic acid hydrazide (ABAH) and indomethacin to the granulocytes, the generation of superoxide anion increased and the decreasing effect of the steroids on superoxide production was inhibited. Incubating the neutrophils with the product of the reaction catalyzed by MPO itself (hypochlorite anion), we found significant decrease in superoxide generation. According to our results MPO seems to diminish the production of superoxide anion and so probably has an antioxidant ability. Therefore, its lower plasma levels may contribute to the increasing incidence of atherosclerosis and other free radical mediated disorders in old people. Thus, after further studies MPO might become one of the indicators of cardiovascular risk and the scavenger capacity in general.

Original languageEnglish
Pages (from-to)137-148
Number of pages12
JournalExperimental Gerontology
Volume37
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Superoxides
Peroxidase
Plasmas
Free Radicals
Enzymes
Granulocytes
Neutrophils
Atherosclerosis
Antioxidants
Steroid hormones
Steroids
Photometry
Hypochlorous Acid
Blood Donors
Indomethacin
Anions
Blood
Enzyme-Linked Immunosorbent Assay
Hormones
Incidence

Keywords

  • Antioxidant effect
  • Atherosclerosis
  • Myeloperoxidase enzyme
  • Myeloperoxidase inhibitors
  • Neutrophil granulocytes
  • Steroids
  • Superoxide anion
  • Vasoprotection

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

Plasma concentration of myeloperoxidase enzyme in pre- and post-climacterial people : Related superoxide anion generation. / Békési, Gábor; Kakucs, Réka; Sándor, József; Sárváry, E.; Kocsis, I.; Sprintz, Detlef; Várbíró, S.; Magyar, Zoltán; Hrabák, A.; Fehér, J.; Székács, B.

In: Experimental Gerontology, Vol. 37, No. 1, 2001, p. 137-148.

Research output: Contribution to journalArticle

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AU - Sárváry, E.

AU - Kocsis, I.

AU - Sprintz, Detlef

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