Plasma adrenocorticotropin in domestic geese

Effects of ether stress and endocrine manipulations

K. Kovács, Péter Péczely

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A direct radioimmunoassay for the determination of avian adrenocorticotropin (ACTH) in a small volume of plasma was developed using an antiserum specific for N-terminal region of the ACTH molecule. The sensitivity of the two stage assay is 0.1 fmol ACTH/tube. The specificity of the antiserum was tested by its cross reactions with synthetic ACTH fragments and by comparing curves obtained by dilution of different plasma specimens to that of ACTH1-39 reference standard. Adrenocorticotropin responses of chronically cannulated geese to ether stress were evaluated and compared to changes of plasma corticosterone (B) concentration over a 2-hr period. ACTH showed a maximum between 5 and 10 min after ether exposure, while B peak appeared 10-15 min later. Thirty minutes after ether inhalation plasma ACTH returned to the baseline, while B response was longer-lasting and decreased to the resting level between 60 and 120 min. Basal and stress-induced ACTH plasma levels were also investigated in male and female gonadectomized and thyroidectomized geese. Castration increased, while thyroidectomy decreased the basal ACTH concentration. These endocrine manipulations did not, however, markedly affect the stress-induced ACTH hypersecretion except in thyroidectomized ganders, where the increment of plasma ACTH 10 min after ether inhalation was significantly lower than in sham operated control geese.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume84
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Geese
corticotropin
geese
Ether
Adrenocorticotropic Hormone
ethers
Inhalation
Immune Sera
breathing
antiserum
ganders
Cosyntropin
hypersecretion
Plasma Volume
Castration
Cross Reactions
Thyroidectomy
Corticosterone
reference standards
corticosterone

ASJC Scopus subject areas

  • Endocrinology

Cite this

Plasma adrenocorticotropin in domestic geese : Effects of ether stress and endocrine manipulations. / Kovács, K.; Péczely, Péter.

In: General and Comparative Endocrinology, Vol. 84, No. 2, 1991, p. 192-198.

Research output: Contribution to journalArticle

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