Plaque-based competitive hybridization

Zoltán Villányi, István Gyurján, Viktor Stéger, László Orosz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The authors have developed a simple, cost-saving experimental design, plaque-based competitive hybridization (PBCH), for genome-wide identification of genes differentially expressed in different tissues. PBCH offers advantages in comparison with other methods used in comparative genomics by combining the principles of differential hybridization with the subtractive hybridization. PBCH is particularly advantageous when libraries with few differences are to be analyzed. The authors demonstrate the use of PBCH by identifying 3 genes, up-regulated in the developing velvet antler of red deer (Cervus elaphus): ApoD, C011A2, and S100a1. The fidelity and sensitivity of PBCH is also shown: 1 specific clone among a library sample of 15,000 can be recognized. Possibilities for further utilizations are discussed.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalJournal of Biomolecular Screening
Volume13
Issue number1
DOIs
Publication statusPublished - Feb 2008

Keywords

  • Antler
  • Bone
  • Cartilage
  • Deer
  • Library screening

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

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