Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs

Attila Molnár, Tibor Csorba, L. Lakatos, E. Várallyay, Christophe Lacomme, J. Burgyán

Research output: Contribution to journalArticle

274 Citations (Scopus)

Abstract

RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena of RNA interference in animals and posttranscriptional gene silencing (PTGS) in plants. In plants, PTGS acts as an antiviral system; a successful virus infection requires suppression or evasion of the induced silencing response. Small interfering RNAs (siRNAs) accumulate in plants infected with positive-strand RNA viruses and provide specificity to this RNA-mediated defense. We present here the results of a survey of virus-specific siRNAs characterized by a sequence analysis of siRNAs from plants infected with Cymbidium ringspot tombusvirus (CymRSV). CymRSV siRNA sequences have a nonrandom distribution along the length of the viral genome, suggesting that there are hot spots for virus-derived siRNA generation. CymRSV siRNAs bound to the CymRSV p19 suppressor protein have the same asymmetry in strand polarity as the sequenced siRNAs and are imperfect double-stranded RNA duplexes. Moreover, an analysis of siRNAs derived from two other nonrelated positive-strand RNA viruses showed that they displayed the same asymmetry as CymRSV siRNAs. Finally, we show that Tobacco mosaic virus (TMV) carrying a short inverted repeat of the phytoene desaturase (PDS) gene triggered more accumulation of PDS siRNAs than the corresponding antisense PDS sequence. Taken together, these results suggest that virus-derived siRNAs originate predominantly by direct DICER cleavage of imperfect duplexes in the most folded regions of the positive strand of the viral RNA.

Original languageEnglish
Pages (from-to)7812-7818
Number of pages7
JournalJournal of Virology
Volume79
Issue number12
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Plant Viruses
plant viruses
Viral RNA
small interfering RNA
Small Interfering RNA
Cymbidium ringspot virus
Tombusvirus
RNA
RNA Interference
RNA interference
viruses
RNA Viruses
Viruses
Tobacco Mosaic Virus
Plant Genes
Tobacco mosaic virus
Double-Stranded RNA
Viral Genome
double-stranded RNA
Virus Diseases

ASJC Scopus subject areas

  • Immunology

Cite this

Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs. / Molnár, Attila; Csorba, Tibor; Lakatos, L.; Várallyay, E.; Lacomme, Christophe; Burgyán, J.

In: Journal of Virology, Vol. 79, No. 12, 06.2005, p. 7812-7818.

Research output: Contribution to journalArticle

@article{a9a5531d4cc54e6085d2f49688f5cc24,
title = "Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs",
abstract = "RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena of RNA interference in animals and posttranscriptional gene silencing (PTGS) in plants. In plants, PTGS acts as an antiviral system; a successful virus infection requires suppression or evasion of the induced silencing response. Small interfering RNAs (siRNAs) accumulate in plants infected with positive-strand RNA viruses and provide specificity to this RNA-mediated defense. We present here the results of a survey of virus-specific siRNAs characterized by a sequence analysis of siRNAs from plants infected with Cymbidium ringspot tombusvirus (CymRSV). CymRSV siRNA sequences have a nonrandom distribution along the length of the viral genome, suggesting that there are hot spots for virus-derived siRNA generation. CymRSV siRNAs bound to the CymRSV p19 suppressor protein have the same asymmetry in strand polarity as the sequenced siRNAs and are imperfect double-stranded RNA duplexes. Moreover, an analysis of siRNAs derived from two other nonrelated positive-strand RNA viruses showed that they displayed the same asymmetry as CymRSV siRNAs. Finally, we show that Tobacco mosaic virus (TMV) carrying a short inverted repeat of the phytoene desaturase (PDS) gene triggered more accumulation of PDS siRNAs than the corresponding antisense PDS sequence. Taken together, these results suggest that virus-derived siRNAs originate predominantly by direct DICER cleavage of imperfect duplexes in the most folded regions of the positive strand of the viral RNA.",
author = "Attila Moln{\'a}r and Tibor Csorba and L. Lakatos and E. V{\'a}rallyay and Christophe Lacomme and J. Burgy{\'a}n",
year = "2005",
month = "6",
doi = "10.1128/JVI.79.12.7812-7818.2005",
language = "English",
volume = "79",
pages = "7812--7818",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs

AU - Molnár, Attila

AU - Csorba, Tibor

AU - Lakatos, L.

AU - Várallyay, E.

AU - Lacomme, Christophe

AU - Burgyán, J.

PY - 2005/6

Y1 - 2005/6

N2 - RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena of RNA interference in animals and posttranscriptional gene silencing (PTGS) in plants. In plants, PTGS acts as an antiviral system; a successful virus infection requires suppression or evasion of the induced silencing response. Small interfering RNAs (siRNAs) accumulate in plants infected with positive-strand RNA viruses and provide specificity to this RNA-mediated defense. We present here the results of a survey of virus-specific siRNAs characterized by a sequence analysis of siRNAs from plants infected with Cymbidium ringspot tombusvirus (CymRSV). CymRSV siRNA sequences have a nonrandom distribution along the length of the viral genome, suggesting that there are hot spots for virus-derived siRNA generation. CymRSV siRNAs bound to the CymRSV p19 suppressor protein have the same asymmetry in strand polarity as the sequenced siRNAs and are imperfect double-stranded RNA duplexes. Moreover, an analysis of siRNAs derived from two other nonrelated positive-strand RNA viruses showed that they displayed the same asymmetry as CymRSV siRNAs. Finally, we show that Tobacco mosaic virus (TMV) carrying a short inverted repeat of the phytoene desaturase (PDS) gene triggered more accumulation of PDS siRNAs than the corresponding antisense PDS sequence. Taken together, these results suggest that virus-derived siRNAs originate predominantly by direct DICER cleavage of imperfect duplexes in the most folded regions of the positive strand of the viral RNA.

AB - RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena of RNA interference in animals and posttranscriptional gene silencing (PTGS) in plants. In plants, PTGS acts as an antiviral system; a successful virus infection requires suppression or evasion of the induced silencing response. Small interfering RNAs (siRNAs) accumulate in plants infected with positive-strand RNA viruses and provide specificity to this RNA-mediated defense. We present here the results of a survey of virus-specific siRNAs characterized by a sequence analysis of siRNAs from plants infected with Cymbidium ringspot tombusvirus (CymRSV). CymRSV siRNA sequences have a nonrandom distribution along the length of the viral genome, suggesting that there are hot spots for virus-derived siRNA generation. CymRSV siRNAs bound to the CymRSV p19 suppressor protein have the same asymmetry in strand polarity as the sequenced siRNAs and are imperfect double-stranded RNA duplexes. Moreover, an analysis of siRNAs derived from two other nonrelated positive-strand RNA viruses showed that they displayed the same asymmetry as CymRSV siRNAs. Finally, we show that Tobacco mosaic virus (TMV) carrying a short inverted repeat of the phytoene desaturase (PDS) gene triggered more accumulation of PDS siRNAs than the corresponding antisense PDS sequence. Taken together, these results suggest that virus-derived siRNAs originate predominantly by direct DICER cleavage of imperfect duplexes in the most folded regions of the positive strand of the viral RNA.

UR - http://www.scopus.com/inward/record.url?scp=19944370491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944370491&partnerID=8YFLogxK

U2 - 10.1128/JVI.79.12.7812-7818.2005

DO - 10.1128/JVI.79.12.7812-7818.2005

M3 - Article

VL - 79

SP - 7812

EP - 7818

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 12

ER -