Plant tissue culture as a model for study of diversity in formaldehyde binding

I. László, Éva Szoke, Zs Németh, L. Albert

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of endogenous formaldehyde (HCHO) deprivation with dimedone-as abiotic stress-was investigated on the methylation-demethylation reactions in Datura innoxia Mill. callus cultures. The matrix assisted laser desorption/ionization mass spectrometric (MALDI MS) investigation of the culture extracts revealed characteristic differences between the tissues cultivated in light and dark. MALDI MS data show the presence of a precursor molecule and its mono- and tri-hydroxymethyl derivative, only in the cultures maintained in light. The relative amount of the different derivatives changed considerably, as a consequence of dimedone application in the culture medium and during the extraction.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalActa biologica Hungarica
Volume49
Issue number2-4
Publication statusPublished - Dec 1 1998

    Fingerprint

Keywords

  • Datura innoxia Mill.
  • Formaldehyde
  • Hydroxymethyl group
  • Matrix assisted laser desorption/ionization mass spectrometry
  • Methylation-demethylation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this