The effect of endogenous formaldehyde (HCHO) deprivation with dimedone-as abiotic stress-was investigated on the methylation-demethylation reactions in Datura innoxia Mill. callus cultures. The matrix assisted laser desorption/ionization mass spectrometric (MALDI MS) investigation of the culture extracts revealed characteristic differences between the tissues cultivated in light and dark. MALDI MS data show the presence of a precursor molecule and its mono- and tri-hydroxymethyl derivative, only in the cultures maintained in light. The relative amount of the different derivatives changed considerably, as a consequence of dimedone application in the culture medium and during the extraction.
|Number of pages||6|
|Journal||Acta biologica Hungarica|
|Publication status||Published - Dec 1 1998|
- Datura innoxia Mill.
- Hydroxymethyl group
- Matrix assisted laser desorption/ionization mass spectrometry
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Environmental Science(all)