Plant Rho-type (Rop) GTPase-dependent activation of receptor-like cytoplasmic kinases in vitro

Dulguun Dorjgotov, Manuela E. Jurca, Csilla Fodor-Dunai, Attila Szucs, Krisztina Ötvös, Éva Klement, Judit Bíró, Attila Fehér

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly and specifically activated by GTP-bound Rop GTPases in vitro. Deletion analysis indicated that the residues implicated in the interaction might be distributed on various parts of the kinases. Using a chimaeric Rop GTPase protein, the importance of the Rho-insert region in kinase activation could also be verified. These data strengthen the possibility that RLCKs may serve as Rop GTPase effectors in planta.

Original languageEnglish
Pages (from-to)1175-1182
Number of pages8
JournalFEBS letters
Volume583
Issue number7
DOIs
Publication statusPublished - Apr 2 2009

Keywords

  • Arabidopsis thaliana
  • Kinase activity
  • Medicago sp.
  • Protein phosphorylation
  • Protein-protein interaction
  • Signal transduction
  • Small GTP-binding protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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