Plant regeneration of NaCl-pretreated cells from long-term suspension culture of rice (Oryza sativa L.) in high saline conditions

Do Quang Binh, L. Heszky, Gabor Gyulai, Anikó Csillag

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cells of a 2-year-old suspension culture of rice (Oryza sativa L.), grown under 1.5% NaCl stress for 3 months, gave rise to plants through embryogenesis in different saline conditions. The high regeneration potential (59.6%) on salt-free medium decreased rapidly with increasing concentration of salt in the regeneration medium. At 1.25% NaCl, healthy shoots were developed in 14.9% of the cultures. Under 1.5% salt stress, embryo formation and embryo germination (6.1%) was observed but further development into plants was inhibited. Cells not pretreated with salt produced plants at a low frequency (2.6-4.2%) both in salt-free and low saline condition (0.75-1% NaCl). Cells pretreated for 3 months with 0.75% salt did not give rise to plants on all tested media. Plants regenerated from the salt-stressed cultures were transferred to soil and grew to maturity in a greenhouse.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalPlant Cell, Tissue and Organ Culture
Volume29
Issue number2
DOIs
Publication statusPublished - May 1992

Fingerprint

Regeneration
Suspensions
Oryza sativa
Salts
salts
rice
embryo (plant)
cells
Embryonic Structures
salt concentration
salt stress
plant development
Plant Development
embryogenesis
Germination
Oryza
germination
greenhouses
Embryonic Development
shoots

Keywords

  • embryogenesis
  • NaCl-stress

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Cite this

Plant regeneration of NaCl-pretreated cells from long-term suspension culture of rice (Oryza sativa L.) in high saline conditions. / Binh, Do Quang; Heszky, L.; Gyulai, Gabor; Csillag, Anikó.

In: Plant Cell, Tissue and Organ Culture, Vol. 29, No. 2, 05.1992, p. 75-82.

Research output: Contribution to journalArticle

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