Plant regeneration of NaCl-pretreated cells from long-term suspension culture of rice (Oryza sativa L.) in high saline conditions

Do Quang Binh, László E. Heszky, Gabor Gyulai, Anikó Csillag

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cells of a 2-year-old suspension culture of rice (Oryza sativa L.), grown under 1.5% NaCl stress for 3 months, gave rise to plants through embryogenesis in different saline conditions. The high regeneration potential (59.6%) on salt-free medium decreased rapidly with increasing concentration of salt in the regeneration medium. At 1.25% NaCl, healthy shoots were developed in 14.9% of the cultures. Under 1.5% salt stress, embryo formation and embryo germination (6.1%) was observed but further development into plants was inhibited. Cells not pretreated with salt produced plants at a low frequency (2.6-4.2%) both in salt-free and low saline condition (0.75-1% NaCl). Cells pretreated for 3 months with 0.75% salt did not give rise to plants on all tested media. Plants regenerated from the salt-stressed cultures were transferred to soil and grew to maturity in a greenhouse.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalPlant Cell, Tissue and Organ Culture
Volume29
Issue number2
DOIs
Publication statusPublished - May 1 1992

Keywords

  • NaCl-stress
  • embryogenesis

ASJC Scopus subject areas

  • Horticulture

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