Plant regeneration from embryogenic cultures of Phragmites australis (Cav.) Trin. Ex Steud.

Csaba Máthé, Márta M. Hamvas, István Grigorszky, Gábor Vasas, Erika Molnár, J. Brian Power, Michael R. Davey, George Borbély

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Embryogenic cultures of tine common reed [Phragmites australis (Cav.) Trin. Ex. Steud.] were induced on Murashige and Skoog (1962)-based medium with 2% (w/v) sucrose, B5 vitamins and 4.5 μM 2,4-dichlorphenoxyacetic acid. Four independent culture lines, two initiated from stem nodes and two from roots, were established. These cultures underwent somatic embryogenesis. In one line of stem node origin, the somatic embryos germinated and developed into plants, following transfer of embryogenic cultures to Murashige and Skoog (1962)-based medium lacking growth regulators, with 108 ± 17 plants being recovered per 100 mg fresh weight of culture. In other lines, the somatic embryos developed roots, but not shoots. Shoot regeneration via somatic embryogenesis offers potential as an in vitro system for physiological studies, including assessments of the response of common reed to environmental pollutants.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Volume63
Issue number1
DOIs
Publication statusPublished - Dec 1 2000

Keywords

  • Common reed
  • Phragmites australis
  • Plant regeneration
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Horticulture

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