Plant processing by simultaneous lactic acid fermentation and enzyme hydrolysis

R. P. Tengerdy, J. C. Linden, Mei Wu, V. G. Murphy, F. Baintner, G. Szakacs

Research output: Contribution to journalArticle

5 Citations (Scopus)


Traditional ensiling of plant material by anaerobic lactic acid fermentation was combined with enzymatic hydrolysis (ENLAC for short) with cell wall degrading enzymes (hemicellulases, cellulases, and pectinases) to increase fiber digestibility or to increase the recovery of cell content from plants. Such findings were made using 0.015% (w/w, wet basis) Phylacell® enzyme preparation by ENLAC of corn and corn-sorghum mixtures, but not of forage grasses. Addition to alfalfa of a mixture of cell wall degrading enzymes, such as NOVO Viscozyme® together with NOVO Celluclast® each at 0.2-1.0% (w/w, wet basis), resulted in more rapid ensiling and improvement of rumen digestibility of silage by 20%. After 20 d of ensiling at 25 °C when the same enzymes were added to alfalfa at the 1.0% level, protein recovery by pressing increased by 35%, β-carotene recovery by 80%, and chlorophyll/xanthophyll recovery by 30%. ENLAC with the same enzymes also increased the recovery of sclareol from muscatel sage by 400%.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalApplied Biochemistry and Biotechnology
Issue number1
Publication statusPublished - Mar 1 1992


  • Ensiling
  • cell content recovery
  • degradation
  • enzyme hydrolysis
  • plant cell wall

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Plant processing by simultaneous lactic acid fermentation and enzyme hydrolysis'. Together they form a unique fingerprint.

  • Cite this