Plant peptides govern terminal differentiation of bacteria in symbiosis

Willem Van De Velde, Grigor Zehirov, Agnes Szatmari, Monika Debreczeny, Hironobu Ishihara, Zoltan Kevei, Attila Farkas, Kata Mikulass, Andrea Nagy, Hilda Tiricz, Beatrice Satiat-Jeunemaître, Benoit Alunni, Mickael Bourge, Ken Ichi Kucho, Mikiko Abe, Attila Kereszt, Gergely Maroti, Toshiki Uchiumi, Eva Kondorosi, Peter Mergaert

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311 Citations (Scopus)

Abstract

Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicaga truncatula, the bacteria undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides that are targeted to the bacteria and enter the bacterial membrane and cytosol. Obstruction of NCR transport in the dnf1-1 signal peptidase mutant correlated with the absence of terminal bacterial differentiation. On the contrary, ectopic expression of NCRs in legumes devoid of NCRs or challenge of cultured rhizobia with peptides provoked symptoms of terminal differentiation. Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria.

Original languageEnglish
Pages (from-to)1122-1126
Number of pages5
JournalScience
Volume327
Issue number5969
DOIs
Publication statusPublished - Feb 26 2010

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    Van De Velde, W., Zehirov, G., Szatmari, A., Debreczeny, M., Ishihara, H., Kevei, Z., Farkas, A., Mikulass, K., Nagy, A., Tiricz, H., Satiat-Jeunemaître, B., Alunni, B., Bourge, M., Kucho, K. I., Abe, M., Kereszt, A., Maroti, G., Uchiumi, T., Kondorosi, E., & Mergaert, P. (2010). Plant peptides govern terminal differentiation of bacteria in symbiosis. Science, 327(5969), 1122-1126. https://doi.org/10.1126/science.1184057