Planar chromatographic techniques in biomedicine: current status

A. M. Siouffi, E. Mincsovics, E. Tyihák

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In planar chromatography (PLC), the solvent flows through a layer either by means of capillary forces [conventional thin-layer chromatography (TLC)] or by a forced-flow system (over-pressured layer chromatography). Phases and instrumentation currently available are briefly examined. The main applications in biomedicine are reviewed. Although silica gel TLC plates still predominate, interest in other phases is increasing. Unique detection features such as immunostaining are emphasized. Although gas chromatography and high-performance liquid chromatography have superseded TLC in the analysis of carbohydrates, amino acids and indole derivatives, interest in PLC continues to be high in lipid analysis.

Original languageEnglish
Pages (from-to)471-538
Number of pages68
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume492
Issue numberC
DOIs
Publication statusPublished - Aug 8 1989

Fingerprint

Thin layer chromatography
Thin Layer Chromatography
Chromatography
Silica Gel
High performance liquid chromatography
Gas chromatography
Gas Chromatography
Gel Chromatography
High Pressure Liquid Chromatography
Carbohydrates
Derivatives
Lipids
Amino Acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Planar chromatographic techniques in biomedicine : current status. / Siouffi, A. M.; Mincsovics, E.; Tyihák, E.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 492, No. C, 08.08.1989, p. 471-538.

Research output: Contribution to journalArticle

@article{3b9a1896383948d8a486a5313aeab1fe,
title = "Planar chromatographic techniques in biomedicine: current status",
abstract = "In planar chromatography (PLC), the solvent flows through a layer either by means of capillary forces [conventional thin-layer chromatography (TLC)] or by a forced-flow system (over-pressured layer chromatography). Phases and instrumentation currently available are briefly examined. The main applications in biomedicine are reviewed. Although silica gel TLC plates still predominate, interest in other phases is increasing. Unique detection features such as immunostaining are emphasized. Although gas chromatography and high-performance liquid chromatography have superseded TLC in the analysis of carbohydrates, amino acids and indole derivatives, interest in PLC continues to be high in lipid analysis.",
author = "Siouffi, {A. M.} and E. Mincsovics and E. Tyih{\'a}k",
year = "1989",
month = "8",
day = "8",
doi = "10.1016/S0378-4347(00)84478-3",
language = "English",
volume = "492",
pages = "471--538",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "C",

}

TY - JOUR

T1 - Planar chromatographic techniques in biomedicine

T2 - current status

AU - Siouffi, A. M.

AU - Mincsovics, E.

AU - Tyihák, E.

PY - 1989/8/8

Y1 - 1989/8/8

N2 - In planar chromatography (PLC), the solvent flows through a layer either by means of capillary forces [conventional thin-layer chromatography (TLC)] or by a forced-flow system (over-pressured layer chromatography). Phases and instrumentation currently available are briefly examined. The main applications in biomedicine are reviewed. Although silica gel TLC plates still predominate, interest in other phases is increasing. Unique detection features such as immunostaining are emphasized. Although gas chromatography and high-performance liquid chromatography have superseded TLC in the analysis of carbohydrates, amino acids and indole derivatives, interest in PLC continues to be high in lipid analysis.

AB - In planar chromatography (PLC), the solvent flows through a layer either by means of capillary forces [conventional thin-layer chromatography (TLC)] or by a forced-flow system (over-pressured layer chromatography). Phases and instrumentation currently available are briefly examined. The main applications in biomedicine are reviewed. Although silica gel TLC plates still predominate, interest in other phases is increasing. Unique detection features such as immunostaining are emphasized. Although gas chromatography and high-performance liquid chromatography have superseded TLC in the analysis of carbohydrates, amino acids and indole derivatives, interest in PLC continues to be high in lipid analysis.

UR - http://www.scopus.com/inward/record.url?scp=0024394318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024394318&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(00)84478-3

DO - 10.1016/S0378-4347(00)84478-3

M3 - Article

C2 - 2671001

AN - SCOPUS:0024394318

VL - 492

SP - 471

EP - 538

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - C

ER -