Pka values and partition coefficients of nitroxide spin probes for membrane bioenergetics measurements

Jürgen Fuchs, Wolfgang H. Nitschmann, Lester Packer, Olga H. Hankovszky, K. Hideg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Knowledge of pKa's is necessary to calculate intracellular/intravesicular pH values from nitroxide accumulation in cells or vesicles as detected with electron spin resonance (ESR) spectroscopy. pKa values were confirmed in lipid vesicles of known internal pH. To help select probes that do not accumulate in lipid membranes, octanol/buffer partition coefficients of uncharged nitroxides were determined. As an application of selected probes, pH gradients and internal aqueous volumes were analyzed in mitochondria (one internal compartment) and in the cyanobacterium Synechococcus 6311 (two internal compartments). The combination of 3-carboxy-, 3-amino- and 3-aminocarbonyl-2,2,5,5-tetramethylpyrrolidin-1-yloxyl was found to be most satisfactory for determinations of internal pH and volumes.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalFree Radical Research
Volume10
Issue number6
DOIs
Publication statusPublished - 1990

Fingerprint

Energy Metabolism
Electron spin resonance spectroscopy
Membranes
Octanols
Mitochondria
Membrane Lipids
Synechococcus
Buffers
Proton-Motive Force
Electron Spin Resonance Spectroscopy
Cyanobacteria
Lipids

Keywords

  • Dissociation constants
  • Mitochondria
  • Nitroxides
  • Partition coefficients
  • PH indicators
  • Synechococcus

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pka values and partition coefficients of nitroxide spin probes for membrane bioenergetics measurements. / Fuchs, Jürgen; Nitschmann, Wolfgang H.; Packer, Lester; Hankovszky, Olga H.; Hideg, K.

In: Free Radical Research, Vol. 10, No. 6, 1990, p. 315-323.

Research output: Contribution to journalArticle

Fuchs, Jürgen ; Nitschmann, Wolfgang H. ; Packer, Lester ; Hankovszky, Olga H. ; Hideg, K. / Pka values and partition coefficients of nitroxide spin probes for membrane bioenergetics measurements. In: Free Radical Research. 1990 ; Vol. 10, No. 6. pp. 315-323.
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