PKC-dependent reduction of the acetylcholine-evoked inward Na current in Aplysia D-neurons: Effect of injected PKC and PKC activators

A. Papp, Jörg Hoyer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of elevated PKC activity on the membrane depolarization (D-response) evoked by extracellular ACh, applied on the soma of Aplysia neurons, was studied. Intracellularly injected PKC and certain PKC activators were used to elevate PKC activity. ACh-induced current was measured in voltage clamp. The neurons were treated extracellularly with the PKC activators: PDAc, SC-10, R-59949, (-)-ILV; or with purified PKC injected into the neuron through the recording electrode. PKC injection and treatment with any of the PKC activators caused a similar reduction of the ACh-induced inward Na current response (corresponding to D-response), while the non-activating α-PDD had no effect. The results provide evidence that a PKC-dependent reduction of receptor responses also exists in this kind of Aplysia neurons. Furthermore, they show that the reduction of ACh response is indeed due to PKC activation (and not to a direct action of the phorbol ester).

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalBrain Research
Volume708
Issue number1-2
DOIs
Publication statusPublished - Feb 5 1996

Fingerprint

Aplysia
Acetylcholine
Neurons
R 59949
Carisoprodol
Phorbol Esters
Electrodes
Injections
Membranes
Therapeutics

Keywords

  • Acetylcholine
  • Acetylcholine receptor
  • Aplysia californica
  • Phorbol ester
  • Protein kinase C
  • Protein kinase C activator

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

PKC-dependent reduction of the acetylcholine-evoked inward Na current in Aplysia D-neurons : Effect of injected PKC and PKC activators. / Papp, A.; Hoyer, Jörg.

In: Brain Research, Vol. 708, No. 1-2, 05.02.1996, p. 123-127.

Research output: Contribution to journalArticle

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