Pituitary adenylate cyclase activating polypeptide (PACAP) pathway is induced by mechanical load and reduces the activity of hedgehog signaling in chondrogenic micromass cell cultures

Tamás Juhász, Eszter Szentléleky, Csilla Szűcs Somogyi, Rol Takács, Nóra Dobrosi, Máté Engler, A. Tamás, D. Reglodi, Róza Zákány

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass cell cultures. Since no data can be found if PACAP signaling is playing any role during mechanical stress in any tissues, we aimed to investigate its contribution in mechanotransduction during chondrogenesis. Expressions of the mRNAs of PACAP and its major receptor, PAC1 increased, while that of other receptors, VPAC1, VPAC2 decreased upon mechanical stimulus. Mechanical load enhanced the expression of collagen type X, a marker of hypertrophic differentiation of chondrocytes and PACAP addition attenuated this elevation. Moreover, exogenous PACAP also prevented the mechanical load evoked activation of hedgehog signaling: protein levels of Sonic and Indian Hedgehogs and Gli1 transcription factor were lowered while expressions of Gli2 and Gli3 were elevated by PACAP application during mechanical load. Our results suggest that mechanical load activates PACAP signaling and exogenous PACAP acts against the hypertrophy inducing effect of mechanical load.

Original languageEnglish
Pages (from-to)17344-17367
Number of pages24
JournalInternational Journal of Molecular Sciences
Volume16
Issue number8
DOIs
Publication statusPublished - Jul 29 2015

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Hedgehogs
polypeptides
Cell culture
Cell Culture Techniques
Receptors, Vasoactive Intestinal Peptide, Type II
Receptors, Vasoactive Intestinal Polypeptide, Type I
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Hedgehog Proteins
Collagen Type X
Tissue
Limb Buds
Chondrogenesis
chickens
Mechanical Stress
Transcription factors
Differentiation Antigens
collagens
Chondrocytes
limbs

Keywords

  • Chondrocyte differentiation
  • Hypertrophy
  • Indian hedgehog
  • Mechanotransduction
  • Sonic hedgehog

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

Cite this

Pituitary adenylate cyclase activating polypeptide (PACAP) pathway is induced by mechanical load and reduces the activity of hedgehog signaling in chondrogenic micromass cell cultures. / Juhász, Tamás; Szentléleky, Eszter; Somogyi, Csilla Szűcs; Takács, Rol; Dobrosi, Nóra; Engler, Máté; Tamás, A.; Reglodi, D.; Zákány, Róza.

In: International Journal of Molecular Sciences, Vol. 16, No. 8, 29.07.2015, p. 17344-17367.

Research output: Contribution to journalArticle

Juhász, Tamás ; Szentléleky, Eszter ; Somogyi, Csilla Szűcs ; Takács, Rol ; Dobrosi, Nóra ; Engler, Máté ; Tamás, A. ; Reglodi, D. ; Zákány, Róza. / Pituitary adenylate cyclase activating polypeptide (PACAP) pathway is induced by mechanical load and reduces the activity of hedgehog signaling in chondrogenic micromass cell cultures. In: International Journal of Molecular Sciences. 2015 ; Vol. 16, No. 8. pp. 17344-17367.
@article{56bb5934ef064e41ba0a390389f1d007,
title = "Pituitary adenylate cyclase activating polypeptide (PACAP) pathway is induced by mechanical load and reduces the activity of hedgehog signaling in chondrogenic micromass cell cultures",
abstract = "Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass cell cultures. Since no data can be found if PACAP signaling is playing any role during mechanical stress in any tissues, we aimed to investigate its contribution in mechanotransduction during chondrogenesis. Expressions of the mRNAs of PACAP and its major receptor, PAC1 increased, while that of other receptors, VPAC1, VPAC2 decreased upon mechanical stimulus. Mechanical load enhanced the expression of collagen type X, a marker of hypertrophic differentiation of chondrocytes and PACAP addition attenuated this elevation. Moreover, exogenous PACAP also prevented the mechanical load evoked activation of hedgehog signaling: protein levels of Sonic and Indian Hedgehogs and Gli1 transcription factor were lowered while expressions of Gli2 and Gli3 were elevated by PACAP application during mechanical load. Our results suggest that mechanical load activates PACAP signaling and exogenous PACAP acts against the hypertrophy inducing effect of mechanical load.",
keywords = "Chondrocyte differentiation, Hypertrophy, Indian hedgehog, Mechanotransduction, Sonic hedgehog",
author = "Tam{\'a}s Juh{\'a}sz and Eszter Szentl{\'e}leky and Somogyi, {Csilla Szűcs} and Rol Tak{\'a}cs and N{\'o}ra Dobrosi and M{\'a}t{\'e} Engler and A. Tam{\'a}s and D. Reglodi and R{\'o}za Z{\'a}k{\'a}ny",
year = "2015",
month = "7",
day = "29",
doi = "10.3390/ijms160817344",
language = "English",
volume = "16",
pages = "17344--17367",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "8",

}

TY - JOUR

T1 - Pituitary adenylate cyclase activating polypeptide (PACAP) pathway is induced by mechanical load and reduces the activity of hedgehog signaling in chondrogenic micromass cell cultures

AU - Juhász, Tamás

AU - Szentléleky, Eszter

AU - Somogyi, Csilla Szűcs

AU - Takács, Rol

AU - Dobrosi, Nóra

AU - Engler, Máté

AU - Tamás, A.

AU - Reglodi, D.

AU - Zákány, Róza

PY - 2015/7/29

Y1 - 2015/7/29

N2 - Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass cell cultures. Since no data can be found if PACAP signaling is playing any role during mechanical stress in any tissues, we aimed to investigate its contribution in mechanotransduction during chondrogenesis. Expressions of the mRNAs of PACAP and its major receptor, PAC1 increased, while that of other receptors, VPAC1, VPAC2 decreased upon mechanical stimulus. Mechanical load enhanced the expression of collagen type X, a marker of hypertrophic differentiation of chondrocytes and PACAP addition attenuated this elevation. Moreover, exogenous PACAP also prevented the mechanical load evoked activation of hedgehog signaling: protein levels of Sonic and Indian Hedgehogs and Gli1 transcription factor were lowered while expressions of Gli2 and Gli3 were elevated by PACAP application during mechanical load. Our results suggest that mechanical load activates PACAP signaling and exogenous PACAP acts against the hypertrophy inducing effect of mechanical load.

AB - Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass cell cultures. Since no data can be found if PACAP signaling is playing any role during mechanical stress in any tissues, we aimed to investigate its contribution in mechanotransduction during chondrogenesis. Expressions of the mRNAs of PACAP and its major receptor, PAC1 increased, while that of other receptors, VPAC1, VPAC2 decreased upon mechanical stimulus. Mechanical load enhanced the expression of collagen type X, a marker of hypertrophic differentiation of chondrocytes and PACAP addition attenuated this elevation. Moreover, exogenous PACAP also prevented the mechanical load evoked activation of hedgehog signaling: protein levels of Sonic and Indian Hedgehogs and Gli1 transcription factor were lowered while expressions of Gli2 and Gli3 were elevated by PACAP application during mechanical load. Our results suggest that mechanical load activates PACAP signaling and exogenous PACAP acts against the hypertrophy inducing effect of mechanical load.

KW - Chondrocyte differentiation

KW - Hypertrophy

KW - Indian hedgehog

KW - Mechanotransduction

KW - Sonic hedgehog

UR - http://www.scopus.com/inward/record.url?scp=84938360225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938360225&partnerID=8YFLogxK

U2 - 10.3390/ijms160817344

DO - 10.3390/ijms160817344

M3 - Article

C2 - 26230691

AN - SCOPUS:84938360225

VL - 16

SP - 17344

EP - 17367

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 8

ER -