Pituitary adenylate cyclase-activating polypeptide-like compounds could modulate the activity of coelomocytes in the earthworm

Ildiko Somogyi, Akos Boros, Peter Engelmann, Eszter Varhalmi, Jozsef Nemeth, Andrea Lubics, Andrea Tamas, Peter Kiss, Dora Reglodi, Edit Pollak, Laszlo Molnar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

By means of radioimmunoassay, we studied the concentration of pituitary adenylate cyclase-activating polypeptide (PACAP)-like proteins in intact and regenerating earthworms. Transection of animals increased the concentration of PACAP-like compounds in coelomocytes, and a decreasing rostrocaudal gradient was detected in the regenerating animals. Western blot analysis revealed a range of PAC1-receptor proteins with molecular weights from 40 to 80 kDa. Electron microscopic immunocytochemistry showed that PAC1 receptors were located on distinct sets of coelomocytes (mainly on amebocytes and on some granulocytes). Based on our results we hypothesize a link between PACAP and coelomocytes, suggesting that PACAP modulates the function of amebocytes and certain granulocytes that play a role in tissue remodeling of regenerating earthworms.

Original languageEnglish
Title of host publicationTrends in Comparative Endocrinology and Neurobiology
PublisherBlackwell Publishing Inc.
Pages521-523
Number of pages3
ISBN (Print)9781573316712
DOIs
Publication statusPublished - Apr 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1163
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Eisenia fetida
  • Immunocytochemistry
  • Radioimmunoassay
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Somogyi, I., Boros, A., Engelmann, P., Varhalmi, E., Nemeth, J., Lubics, A., Tamas, A., Kiss, P., Reglodi, D., Pollak, E., & Molnar, L. (2009). Pituitary adenylate cyclase-activating polypeptide-like compounds could modulate the activity of coelomocytes in the earthworm. In Trends in Comparative Endocrinology and Neurobiology (pp. 521-523). (Annals of the New York Academy of Sciences; Vol. 1163). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04431.x