Pituitary adenylate cyclase-activating polypeptide deficiency enhances oxazolone-induced allergic contact dermatitis in mice

Ágnes Kemény, Dóra Reglődi, Renáta Cseharovszky, Hitoshi Hashimoto, Akemichi Baba, János Szolcsányi, Erika Pintér, Zsuzsanna Helyes

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17 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is present in capsaicin-sensitive sensory neurons and inflammatory/immune cells, therefore it is suggested to play a role in neuro-immune interactions. Our aim was to investigate the role of PACAP in oxazolone-induced delayed-type hypersensitivity reaction in the skin using deficient mice (PACAP -/- ). Sensitization was induced by 2% oxazolone application on the shaved abdomen on two consecutive days; inflammation was elicited by oxazolone smearing on the ears 6 days later. Ear thickness was measured by micrometry. Histological examination, cytokine profile [IL-2, IL-4, IL-5, and monocyte chemoattractant protein-1: MCP-1, IFN-γ, tumor necrosis factor alpha (TNF-α)] and myeloperoxidase activity correlating with the number of neutrophils/macrophages were determined 24 and 48 h later. Oxazolone induced a 110-130% swelling after 24-48 h in wild-type mice, which was significantly greater in PACAP-deficient mice. Histological analysis confirmed markedly increased edema in PACAP -/- mice, but the moderately enhanced inflammatory cell accumulation was not statistically significant compared with the wild-types. There was no difference in myeloperoxidase activity of the ear homogenates. Elevation of MCP-1, but not the levels of the other cytokines, was significantly higher in the samples of the PACAP-deficient mice. These results suggest that PACAP exerts anti-inflammatory, particularly edema-inhibiting effects in allergic contact dermatitis.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalJournal of Molecular Neuroscience
Volume42
Issue number3
DOIs
Publication statusPublished - Nov 2010

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Keywords

  • Delayed-type hypersensitivity reaction
  • Ear edema
  • Histopathological alterations
  • Inflammatory cytokines
  • Myeloperoxidase activity

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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