Pigment epithelial cells isolated from human peripheral iridectomies have limited properties of retinal stem cells

Rebecca C. Frøen, Erik O. Johnsen, G. Petrovski, Erika Berényi, Andrea Facskõ, A. Berta, Bjørn Nicolaissen, Morten C. Moe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: The identification of cells with properties of retinal progenitor cells (RPCs) in the adult human ciliary margin (CM) prompted a number of studies of their proliferative and differentiation potential. One of the remaining challenges is to find a feasible method of isolating RPCs from the patient's eye. In the human CM, only the iris pigment epithelium (IPE) is easily obtained by a minimally invasive procedure. In the light of recent studies questioning the existence of RPCs in the adult mammalian CM, we wanted to assess the potential of the adult human IPE as source of RPCs. Methods: The IPE were isolated from peripheral iridectomies during glaucoma surgery, and IPE and ciliary body (CB) epithelium were also isolated from post-mortem tissue. Cells were cultivated in sphere-promoting conditions or as monolayers. Whole-tissue samples, undifferentiated and differentiated cells were studied by immunocytochemistry, RT-PCR and transmission electron microscopy. Results: The adult human IPE, like the CB, expressed markers of RPCs such as Pax6, Sox2 and Nestin in vivo. Both sphere-promoting and monolayer cultures preserved this phenotype. However, both IPE/CB cultures expressed markers of differentiated epithelial cells such as Claudin, microphtalmia-associated transcription factor (MITF) and Cytokeratin-19. Ultrastructurally, IPE spheres displayed epithelial-like junctions and contained mature melanosomes. After induced differentiation, IPE-derived cells showed only partial neuronal differentiation expressing β-III-tubulin, Map-2 and Rhodopsin, whereas no mature glial markers were found. Conclusion: Proliferative cells with some properties of RPCs can be isolated from the adult human IPE by peripheral iridectomies. Yet, many cells retain properties of differentiated epithelial cells and lack central properties of somatic stem cells.

Original languageEnglish
JournalActa Ophthalmologica
Volume89
Issue number8
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Iridectomy
Iris
Stem Cells
Epithelium
Epithelial Cells
Ciliary Body
Keratin-19
Melanosomes
Nestin
Adult Stem Cells
Rhodopsin
Tubulin
Transmission Electron Microscopy
Neuroglia
Glaucoma
Transcription Factors
Immunohistochemistry

Keywords

  • adult human
  • ciliary body
  • iris pigment epithelium
  • peripheral iridectomy
  • progenitor cell
  • retinal stem cell

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Pigment epithelial cells isolated from human peripheral iridectomies have limited properties of retinal stem cells. / Frøen, Rebecca C.; Johnsen, Erik O.; Petrovski, G.; Berényi, Erika; Facskõ, Andrea; Berta, A.; Nicolaissen, Bjørn; Moe, Morten C.

In: Acta Ophthalmologica, Vol. 89, No. 8, 12.2011.

Research output: Contribution to journalArticle

Frøen, Rebecca C. ; Johnsen, Erik O. ; Petrovski, G. ; Berényi, Erika ; Facskõ, Andrea ; Berta, A. ; Nicolaissen, Bjørn ; Moe, Morten C. / Pigment epithelial cells isolated from human peripheral iridectomies have limited properties of retinal stem cells. In: Acta Ophthalmologica. 2011 ; Vol. 89, No. 8.
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abstract = "Purpose: The identification of cells with properties of retinal progenitor cells (RPCs) in the adult human ciliary margin (CM) prompted a number of studies of their proliferative and differentiation potential. One of the remaining challenges is to find a feasible method of isolating RPCs from the patient's eye. In the human CM, only the iris pigment epithelium (IPE) is easily obtained by a minimally invasive procedure. In the light of recent studies questioning the existence of RPCs in the adult mammalian CM, we wanted to assess the potential of the adult human IPE as source of RPCs. Methods: The IPE were isolated from peripheral iridectomies during glaucoma surgery, and IPE and ciliary body (CB) epithelium were also isolated from post-mortem tissue. Cells were cultivated in sphere-promoting conditions or as monolayers. Whole-tissue samples, undifferentiated and differentiated cells were studied by immunocytochemistry, RT-PCR and transmission electron microscopy. Results: The adult human IPE, like the CB, expressed markers of RPCs such as Pax6, Sox2 and Nestin in vivo. Both sphere-promoting and monolayer cultures preserved this phenotype. However, both IPE/CB cultures expressed markers of differentiated epithelial cells such as Claudin, microphtalmia-associated transcription factor (MITF) and Cytokeratin-19. Ultrastructurally, IPE spheres displayed epithelial-like junctions and contained mature melanosomes. After induced differentiation, IPE-derived cells showed only partial neuronal differentiation expressing β-III-tubulin, Map-2 and Rhodopsin, whereas no mature glial markers were found. Conclusion: Proliferative cells with some properties of RPCs can be isolated from the adult human IPE by peripheral iridectomies. Yet, many cells retain properties of differentiated epithelial cells and lack central properties of somatic stem cells.",
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AU - Berényi, Erika

AU - Facskõ, Andrea

AU - Berta, A.

AU - Nicolaissen, Bjørn

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