Phytoextraction Potential of Wild Type and 35S-GSHI Transgenic Poplar Trees (Populus × Canescens) for Environmental Pollutants Herbicide Paraquat, Salt Sodium, Zinc Sulfate and Nitric Oxide In Vitro

G. Gyulai, A. Bittsánszky, Z. Szabó, Jr Waters, G. Gullner, G. Kampfl, G. Heltai, T. Kőmíves

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Phytoextraction potentials of two transgenic (TR) poplar (Populus × canescens) clones TRggs11 and TRlgl6 were compared with that of wild-type (WT) following exposure to paraquat, zinc sulfate, common salt and nitric oxide (NO), using a leaf-disc system incubated for 21 days on EDTA-containing nutritive WPM media in vitro. Glutathione (GSH) contents of leaf discs of TRlgl6 and TRggs11 showed increments to 296% and 190%, respectively, compared with WT. NO exposure led to a twofold GSH content in TRlgl6, which was coupled with a significantly increased sulfate uptake when exposed to 10-3 M ZnSO4. The highest mineral contents of Na, Zn, Mn, Cu, and Mo was observed in the TRggs11 clone. Salt-induced activity of catalase enzyme increased in both TR clones significantly compared with WT under NaCl (0.75% and 1.5%) exposure. The in silico sequence analyses of gsh1 genes revealed that P. × canadensis and Salix sachalinensis show the closest sequence similarity to that of P. × canescens, which predicted an active GSH production with high phytoextraction potentials of these species with indication for their use where P. × canescens can not be grown.

Original languageEnglish
Pages (from-to)379-396
Number of pages18
JournalInternational Journal of Phytoremediation
Volume16
Issue number4
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Populus canescens
Zinc Sulfate
paraquat
Environmental Pollutants
Paraquat
zinc sulfate
Herbicides
Nitric oxide
phytoremediation
nitric oxide
herbicide
clone
Nitric Oxide
Zinc
herbicides
Salts
pollutants
zinc
Sodium
sodium

Keywords

  • environmental pollutants
  • gene sequence analyses
  • Phytoextraction

ASJC Scopus subject areas

  • Plant Science
  • Environmental Chemistry
  • Pollution

Cite this

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title = "Phytoextraction Potential of Wild Type and 35S-GSHI Transgenic Poplar Trees (Populus × Canescens) for Environmental Pollutants Herbicide Paraquat, Salt Sodium, Zinc Sulfate and Nitric Oxide In Vitro",
abstract = "Phytoextraction potentials of two transgenic (TR) poplar (Populus × canescens) clones TRggs11 and TRlgl6 were compared with that of wild-type (WT) following exposure to paraquat, zinc sulfate, common salt and nitric oxide (NO), using a leaf-disc system incubated for 21 days on EDTA-containing nutritive WPM media in vitro. Glutathione (GSH) contents of leaf discs of TRlgl6 and TRggs11 showed increments to 296{\%} and 190{\%}, respectively, compared with WT. NO exposure led to a twofold GSH content in TRlgl6, which was coupled with a significantly increased sulfate uptake when exposed to 10-3 M ZnSO4. The highest mineral contents of Na, Zn, Mn, Cu, and Mo was observed in the TRggs11 clone. Salt-induced activity of catalase enzyme increased in both TR clones significantly compared with WT under NaCl (0.75{\%} and 1.5{\%}) exposure. The in silico sequence analyses of gsh1 genes revealed that P. × canadensis and Salix sachalinensis show the closest sequence similarity to that of P. × canescens, which predicted an active GSH production with high phytoextraction potentials of these species with indication for their use where P. × canescens can not be grown.",
keywords = "environmental pollutants, gene sequence analyses, Phytoextraction",
author = "G. Gyulai and A. Bitts{\'a}nszky and Z. Szab{\'o} and Jr Waters and G. Gullner and G. Kampfl and G. Heltai and T. Kőm{\'i}ves",
year = "2014",
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T1 - Phytoextraction Potential of Wild Type and 35S-GSHI Transgenic Poplar Trees (Populus × Canescens) for Environmental Pollutants Herbicide Paraquat, Salt Sodium, Zinc Sulfate and Nitric Oxide In Vitro

AU - Gyulai, G.

AU - Bittsánszky, A.

AU - Szabó, Z.

AU - Waters, Jr

AU - Gullner, G.

AU - Kampfl, G.

AU - Heltai, G.

AU - Kőmíves, T.

PY - 2014/4

Y1 - 2014/4

N2 - Phytoextraction potentials of two transgenic (TR) poplar (Populus × canescens) clones TRggs11 and TRlgl6 were compared with that of wild-type (WT) following exposure to paraquat, zinc sulfate, common salt and nitric oxide (NO), using a leaf-disc system incubated for 21 days on EDTA-containing nutritive WPM media in vitro. Glutathione (GSH) contents of leaf discs of TRlgl6 and TRggs11 showed increments to 296% and 190%, respectively, compared with WT. NO exposure led to a twofold GSH content in TRlgl6, which was coupled with a significantly increased sulfate uptake when exposed to 10-3 M ZnSO4. The highest mineral contents of Na, Zn, Mn, Cu, and Mo was observed in the TRggs11 clone. Salt-induced activity of catalase enzyme increased in both TR clones significantly compared with WT under NaCl (0.75% and 1.5%) exposure. The in silico sequence analyses of gsh1 genes revealed that P. × canadensis and Salix sachalinensis show the closest sequence similarity to that of P. × canescens, which predicted an active GSH production with high phytoextraction potentials of these species with indication for their use where P. × canescens can not be grown.

AB - Phytoextraction potentials of two transgenic (TR) poplar (Populus × canescens) clones TRggs11 and TRlgl6 were compared with that of wild-type (WT) following exposure to paraquat, zinc sulfate, common salt and nitric oxide (NO), using a leaf-disc system incubated for 21 days on EDTA-containing nutritive WPM media in vitro. Glutathione (GSH) contents of leaf discs of TRlgl6 and TRggs11 showed increments to 296% and 190%, respectively, compared with WT. NO exposure led to a twofold GSH content in TRlgl6, which was coupled with a significantly increased sulfate uptake when exposed to 10-3 M ZnSO4. The highest mineral contents of Na, Zn, Mn, Cu, and Mo was observed in the TRggs11 clone. Salt-induced activity of catalase enzyme increased in both TR clones significantly compared with WT under NaCl (0.75% and 1.5%) exposure. The in silico sequence analyses of gsh1 genes revealed that P. × canadensis and Salix sachalinensis show the closest sequence similarity to that of P. × canescens, which predicted an active GSH production with high phytoextraction potentials of these species with indication for their use where P. × canescens can not be grown.

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