Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line

Corina Danciu, Delia Muntean, Ersilia Alexa, Claudia Farcas, Camelia Oprean, I. Zupkó, Andrea Bor, Daliana Minda, Maria Proks, Valentina Buda, Monica Hancianu, Oana Cioanca, Codruta Soica, Sofia Popescu, Cristina Adriana Dehelean

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ephedra alata Decne. belongs to the Ephedraceae family. It is a species of Ephedra that grows mostly in the desert. Today, the main importance of Ephedra species in the medical field is due to the presence of the alkaloids derived from phenyl-alanine, which act on the sympathetic nervous system as a sympathomimetic. The aim of this study was to conduct a phytochemical characterization of the hydroalcoholic extract of the aerial part of Ephedra alata Decne., which is indigenous to Tunis, that involves the total phenolic content, individual phenolic content, and antioxidant activity as well as a biological screening for the evaluation of the antimicrobial, antifungal, antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 breast cancer cell line. The results show that the hydroalcoholic extract contains polyphenolic phytocompounds (156.226 ± 0.5 mgGAE/g extract) and elicits antioxidant activity (7453.18 ± 2.5 μmol Trolox/g extract). The extract acted as a bacteriostatic agent against all tested bacterial strains, but was bactericidal only against the Gram-positive cocci and Candida spp. In the set experimental parameters, the extract presents antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 human breast cancer cell line.

Original languageEnglish
JournalMolecules (Basel, Switzerland)
Volume24
Issue number1
DOIs
Publication statusPublished - Dec 20 2018

Fingerprint

Ephedra
antioxidants
Phytochemicals
cultured cells
breast
sympathetic nervous system
Antioxidants
cancer
Cells
adrenergics
Breast Neoplasms
Cell Line
Sympathomimetics
alkaloids
phenylalanine
evaluation
Candida
deserts
Neurology
Alkaloids

Keywords

  • antioxidant
  • bacteria
  • Ephedra alata Decne
  • fungi
  • MCF-7 human breast cancer cell line
  • polyphenols

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line. / Danciu, Corina; Muntean, Delia; Alexa, Ersilia; Farcas, Claudia; Oprean, Camelia; Zupkó, I.; Bor, Andrea; Minda, Daliana; Proks, Maria; Buda, Valentina; Hancianu, Monica; Cioanca, Oana; Soica, Codruta; Popescu, Sofia; Dehelean, Cristina Adriana.

In: Molecules (Basel, Switzerland), Vol. 24, No. 1, 20.12.2018.

Research output: Contribution to journalArticle

Danciu, Corina ; Muntean, Delia ; Alexa, Ersilia ; Farcas, Claudia ; Oprean, Camelia ; Zupkó, I. ; Bor, Andrea ; Minda, Daliana ; Proks, Maria ; Buda, Valentina ; Hancianu, Monica ; Cioanca, Oana ; Soica, Codruta ; Popescu, Sofia ; Dehelean, Cristina Adriana. / Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line. In: Molecules (Basel, Switzerland). 2018 ; Vol. 24, No. 1.
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abstract = "Ephedra alata Decne. belongs to the Ephedraceae family. It is a species of Ephedra that grows mostly in the desert. Today, the main importance of Ephedra species in the medical field is due to the presence of the alkaloids derived from phenyl-alanine, which act on the sympathetic nervous system as a sympathomimetic. The aim of this study was to conduct a phytochemical characterization of the hydroalcoholic extract of the aerial part of Ephedra alata Decne., which is indigenous to Tunis, that involves the total phenolic content, individual phenolic content, and antioxidant activity as well as a biological screening for the evaluation of the antimicrobial, antifungal, antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 breast cancer cell line. The results show that the hydroalcoholic extract contains polyphenolic phytocompounds (156.226 ± 0.5 mgGAE/g extract) and elicits antioxidant activity (7453.18 ± 2.5 μmol Trolox/g extract). The extract acted as a bacteriostatic agent against all tested bacterial strains, but was bactericidal only against the Gram-positive cocci and Candida spp. In the set experimental parameters, the extract presents antiproliferative, pro-apoptotic, and cytotoxic potential against the MCF-7 human breast cancer cell line.",
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AU - Muntean, Delia

AU - Alexa, Ersilia

AU - Farcas, Claudia

AU - Oprean, Camelia

AU - Zupkó, I.

AU - Bor, Andrea

AU - Minda, Daliana

AU - Proks, Maria

AU - Buda, Valentina

AU - Hancianu, Monica

AU - Cioanca, Oana

AU - Soica, Codruta

AU - Popescu, Sofia

AU - Dehelean, Cristina Adriana

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