Physicochemical characterization of stealth liposomes encapsulating an organophosphate hydrolyzing enzyme

M. Budai, Patricia Chapela, Pl Grf, Andreas Zimmer, Melinda E. Wales, James R. Wild, I. Klebovich, Ilona Petrikovics, Mria Szilasi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The present studies were focused on the preparation and characterization of stericaly stabilized liposomes (SLs) encapsulating a recombinant organophosphorus hydrolyzing phosphotriesterase (OPH) enzyme for the antagonism of organophosphorus intoxication. Earlier results indicate that the liposomal carrier system provides an enhanced protective effect against the organophosphorus molecule paraoxon, presenting a more effective therapy with less toxicity than the most commonly used antidotes. Physicochemical characterization of the liposomal OPH delivery system is essential in order to get information on its in vitro stability and in vivo fate. Osmolarity, pH, viscosity, and encapsulation efficiency of the SL preparation and the surface potential of the vesicles were determined. The membrane rigidity and the impact of OPH enzyme on it was studied by electron-paramagnetic resonance spectroscopy, using spin probes. The in vitro stability of the liposomal preparations, the vesicle size distribution, and its alteration during a 3-week storage were followed by dynamic light-scattering measurements. Further, the stability of encapsulated and nonencapsulated OPH was compared in puffer and plasma.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalJournal of Liposome Research
Volume19
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Phosphoric Triester Hydrolases
Organophosphates
Liposomes
Enzymes
Tetraodontiformes
Paraoxon
Antidotes
Electron Spin Resonance Spectroscopy
Viscosity
Osmolar Concentration
Spectrum Analysis
Membranes

Keywords

  • Drug delivery
  • Dynamic light scattering
  • Electron paramagnetic resonance spectroscopy
  • Enzyme-containing stealth liposome
  • Organophosphorus antagonism

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine(all)

Cite this

Physicochemical characterization of stealth liposomes encapsulating an organophosphate hydrolyzing enzyme. / Budai, M.; Chapela, Patricia; Grf, Pl; Zimmer, Andreas; Wales, Melinda E.; Wild, James R.; Klebovich, I.; Petrikovics, Ilona; Szilasi, Mria.

In: Journal of Liposome Research, Vol. 19, No. 2, 2009, p. 163-168.

Research output: Contribution to journalArticle

Budai, M. ; Chapela, Patricia ; Grf, Pl ; Zimmer, Andreas ; Wales, Melinda E. ; Wild, James R. ; Klebovich, I. ; Petrikovics, Ilona ; Szilasi, Mria. / Physicochemical characterization of stealth liposomes encapsulating an organophosphate hydrolyzing enzyme. In: Journal of Liposome Research. 2009 ; Vol. 19, No. 2. pp. 163-168.
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